OBJECTIVE: The aim of this study was to investigate the effects of nano- or submicro topography and fluoride ion on the biology of osteoblasts. STUDY DESIGN: Pure Ti plates were sandblasted, etched with an HCl/H(2)SO(4) solution (control surface) and then etched in a diluted HF/HNO(3) solution (test surface). MC3T3-E1 cells attached, spread, and proliferated on both surfaces. RESULTS: The alkaline phosphatase activity was evidently higher for the test surface than for control surface after 4 and 7 days of cell culture. Real-time PCR showed significant increases in type I collagen and osteocalcin gene expression in osteoblast growth on the test surface after 4 days of culture compared with the control surface. CONCLUSION: With nanotopography and fluoride, hydrogen ions might improve MC3T3-E1 cell proliferation and differentiation during the early stages of cell culture.
OBJECTIVE: The aim of this study was to investigate the effects of nano- or submicro topography and fluoride ion on the biology of osteoblasts. STUDY DESIGN: Pure Ti plates were sandblasted, etched with an HCl/H(2)SO(4) solution (control surface) and then etched in a diluted HF/HNO(3) solution (test surface). MC3T3-E1 cells attached, spread, and proliferated on both surfaces. RESULTS: The alkaline phosphatase activity was evidently higher for the test surface than for control surface after 4 and 7 days of cell culture. Real-time PCR showed significant increases in type I collagen and osteocalcin gene expression in osteoblast growth on the test surface after 4 days of culture compared with the control surface. CONCLUSION: With nanotopography and fluoride, hydrogen ions might improve MC3T3-E1 cell proliferation and differentiation during the early stages of cell culture.