AIMS: Although the aetiology of varicose veins remains unknown, recent studies have focused on endothelial cell integrity and function. Among the regulatory factors of vessel tone, synthesises, pro- and anti-inflammatory, adhesion molecules and the transcription factor hypoxia inducible factor-1 alpha (HIF-1alpha), which are responsible for recruiting leukocytes, are very important. METHODS: Investigation in this study focused on the expression of ICAM-1, E-selectin and HIF-1alpha on endothelial cells using immunostaining and RT-PCR in varicose vein specimens compared with controls. RESULTS: Findings of this study showed alterations of the intima, such as focal intimal discontinuity and denudation of endothelium in varicose veins. Based on data derived from immunostaining and RT-PCR, no major differences were identified between ICAM-1 and E-selectin expression in varicose vein specimens compared with controls. In contrast, immunostaining results identified HIF-1alpha expression in five (5/20) varicose vein specimens, whereas no control saphenous vein specimens expressed HIF-1alpha. CONCLUSIONS: These findings could explain other evidence of hypoxia in varicose veins. Finally, results already obtained in this investigation suggest that the process of pathogenesis of varicose veins is not restricted to the role of adhesion molecules.
AIMS: Although the aetiology of varicose veins remains unknown, recent studies have focused on endothelial cell integrity and function. Among the regulatory factors of vessel tone, synthesises, pro- and anti-inflammatory, adhesion molecules and the transcription factor hypoxia inducible factor-1 alpha (HIF-1alpha), which are responsible for recruiting leukocytes, are very important. METHODS: Investigation in this study focused on the expression of ICAM-1, E-selectin and HIF-1alpha on endothelial cells using immunostaining and RT-PCR in varicose vein specimens compared with controls. RESULTS: Findings of this study showed alterations of the intima, such as focal intimal discontinuity and denudation of endothelium in varicose veins. Based on data derived from immunostaining and RT-PCR, no major differences were identified between ICAM-1 and E-selectin expression in varicose vein specimens compared with controls. In contrast, immunostaining results identified HIF-1alpha expression in five (5/20) varicose vein specimens, whereas no control saphenous vein specimens expressed HIF-1alpha. CONCLUSIONS: These findings could explain other evidence of hypoxia in varicose veins. Finally, results already obtained in this investigation suggest that the process of pathogenesis of varicose veins is not restricted to the role of adhesion molecules.
Authors: Alexandra Shadrina; Elena Voronina; Mariya Smetanina; Yakov Tsepilov; Kseniya Sevost'ianova; Andrey Shevela; Evgenii Seliverstov; Elena Zakharova; Evgeny Ilyukhin; Alexander Kirienko; Igor Zolotukhin; Maxim Filipenko Journal: Immunol Res Date: 2018-02 Impact factor: 2.829
Authors: Chung S Lim; Xiaoying Qiao; Ossama M Reslan; Yin Xia; Joseph D Raffetto; Ewa Paleolog; Alun H Davies; Raouf A Khalil Journal: J Vasc Surg Date: 2010-11-23 Impact factor: 4.268
Authors: Alexandra S Shadrina; Elizaveta E Elgaeva; Ian B Stanaway; Gail P Jarvik; Bahram Namjou; Wei-Qi Wei; Joe Glessner; Hakon Hakonarson; Pradeep Suri; Yakov A Tsepilov Journal: PLoS One Date: 2022-05-20 Impact factor: 3.752