OBJECTIVE: To explore the mechanism and the compensation proceeding of thyroid hyperplasia caused by PTU. METHODS: PTU was administered to rats by gavage at a dose of 5.0 mg/kg B. W for 3, 6, 9, 12, 15, 18, 21 days respectively. All animals were sacrificed after the last dosage, and the expression of TPO and Tg in thyroid was detected by RT-PCR. The serum thyroid hormones were measured by chemoluminescence. RESULTS: Compared with the control group, the TPO mRNA levels of PTU groups were increased, while the Tg mRNA levels were decreased significantly. Serum TT3, TT4 in rats treated with PTU demonstrated a descending trend, while serum TSH showed an ascending trend, and the significant differences were observed after 6 days treating with PTU. CONCLUSION: The interference of PTU on thyroid may relate to inhibiton of Tg gene transcription. The enhancement of TPO gene transcription can't compensate the thyroid function sufficiently.
OBJECTIVE: To explore the mechanism and the compensation proceeding of thyroid hyperplasia caused by PTU. METHODS:PTU was administered to rats by gavage at a dose of 5.0 mg/kg B. W for 3, 6, 9, 12, 15, 18, 21 days respectively. All animals were sacrificed after the last dosage, and the expression of TPO and Tg in thyroid was detected by RT-PCR. The serum thyroid hormones were measured by chemoluminescence. RESULTS: Compared with the control group, the TPO mRNA levels of PTU groups were increased, while the Tg mRNA levels were decreased significantly. Serum TT3, TT4 in rats treated with PTU demonstrated a descending trend, while serum TSH showed an ascending trend, and the significant differences were observed after 6 days treating with PTU. CONCLUSION: The interference of PTU on thyroid may relate to inhibiton of Tg gene transcription. The enhancement of TPO gene transcription can't compensate the thyroid function sufficiently.