BACKGROUND: The aim of this study was to analyze genetic alterations in the transformation-progression model of laryngeal tumors. METHODS: Copy number changes of 37 genes were analyzed by multiple ligation-dependent probe amplification (MLPA) in 94 tissue samples. RESULTS: In the smoker normal mucosa group TP53 loss was predominant, whereas in the precursor lesions CDKN2A loss and CDKN2D gain were most frequent. Precursor lesions with progression presented CTNNB1 loss. In the carcinoma group the most common changes were CDKN2A, MLH1, CTNNB1, and CASP6 losses and RECQL4, CCND1, and EMS1 gains. Positive lymph node primary tumors were related to TP53, IL1A, and RB1 losses and STK11 gain. The lymph node metastases differed from their corresponding primary tumor in LMNA, RECQL4, and IGF1R losses, and N33 and CDKN2D gains. CONCLUSIONS: Genetic changes and new key genes were found to be associated with specific steps. We included new steps, not presented in the classic models: normal mucosa tobacco exposed, positive lymph node primary tumor, and corresponding lymph node metastases.
BACKGROUND: The aim of this study was to analyze genetic alterations in the transformation-progression model of laryngeal tumors. METHODS: Copy number changes of 37 genes were analyzed by multiple ligation-dependent probe amplification (MLPA) in 94 tissue samples. RESULTS: In the smoker normal mucosa group TP53 loss was predominant, whereas in the precursor lesions CDKN2A loss and CDKN2D gain were most frequent. Precursor lesions with progression presented CTNNB1 loss. In the carcinoma group the most common changes were CDKN2A, MLH1, CTNNB1, and CASP6 losses and RECQL4, CCND1, and EMS1 gains. Positive lymph node primary tumors were related to TP53, IL1A, and RB1 losses and STK11 gain. The lymph node metastases differed from their corresponding primary tumor in LMNA, RECQL4, and IGF1R losses, and N33 and CDKN2D gains. CONCLUSIONS: Genetic changes and new key genes were found to be associated with specific steps. We included new steps, not presented in the classic models: normal mucosa tobacco exposed, positive lymph node primary tumor, and corresponding lymph node metastases.
Authors: Ilda Patrícia Ribeiro; Francisco Marques; Francisco Caramelo; João Pereira; Miguel Patrício; Hugo Prazeres; José Ferrão; Maria José Julião; Miguel Castelo-Branco; Joana Barbosa de Melo; Isabel Poiares Baptista; Isabel Marques Carreira Journal: Cell Oncol (Dordr) Date: 2013-12-19 Impact factor: 6.730
Authors: Fernando López; César Alvarez-Marcos; Marta Alonso-Guervós; Francisco Domínguez; Carlos Suárez; Mario A Hermsen; José L Llorente Journal: Eur Arch Otorhinolaryngol Date: 2013-04-07 Impact factor: 2.503
Authors: Fernando López; Teresa Sampedro; José L Llorente; Mario Hermsen; César Álvarez-Marcos Journal: Pathol Oncol Res Date: 2016-07-04 Impact factor: 3.201
Authors: Lorea Manterola; Pablo Aguirre; Erika Larrea; María Arestín; Ayman Gaafar; Kepa Elorriaga; Ibai Goicoechea; María Armesto; Marta Fernández-Mercado; Ignacio Zabalza; Juan Carlos López-Duque; Ekhiñe Larruskain; Jon Alexander Sistiaga; Mikel Landa; Aitor Zabala; Francisco Santaolalla; José Antonio Municio; Ángel Ispizua; Juana María García-Pedrero; Juan Pablo Rodrigo; Charles Henderson Lawrie Journal: Sci Rep Date: 2018-04-26 Impact factor: 4.379