Xiaoyan Ding1, Liang Wang, Yuefang Ren, Wei Zheng. 1. Department of Gynecology, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China.
Abstract
OBJECTIVES: To detect specific mitochondrial proteins in eutopic endometrial samples from women with and without endometriosis and to build diagnostic models. DESIGN: Eutopic endometrial samples from women with endometriosis (excluding adenomyosis) and women with benign indications as control were studied by using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry protein-chip technology. After finding the biomarkers, the diagnostic model was evaluated and validated by leave-one cross-validation. SETTING: Collaborative investigation in an academic research environment. PATIENT(S): Twenty-four patients with endometriosis (excluding adenomyosis) and 29 patients with benign indications as control. INTERVENTION(S): Surgical excision of eutopic endometrial biopsy of patients with endometriosis and controls. MAIN OUTCOME MEASURE(S): Mitochondrial protein expression. RESULT(S): Seventy-eight qualified mitochondrial protein peaks were detected, ten of them had a significant difference. Three combined potential biomarkers, with mass-to-charge ratios (m/z) of 15,334, 15,128, and 16,069, were found, and the diagnostic system distinguished endometriosis from control samples with a specificity of 86.2% and a sensitivity of 87.5%. CONCLUSION(S): We discovered potential mitochondrial biomarkers of eutopic endometrium in endometriosis and set up a diagnostic model. Further identification of the proteins we found will help to explain pathology, new diagnoses, and therapeutic approaches for endometriosis. Crown
OBJECTIVES: To detect specific mitochondrial proteins in eutopic endometrial samples from women with and without endometriosis and to build diagnostic models. DESIGN: Eutopic endometrial samples from women with endometriosis (excluding adenomyosis) and women with benign indications as control were studied by using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry protein-chip technology. After finding the biomarkers, the diagnostic model was evaluated and validated by leave-one cross-validation. SETTING: Collaborative investigation in an academic research environment. PATIENT(S): Twenty-four patients with endometriosis (excluding adenomyosis) and 29 patients with benign indications as control. INTERVENTION(S): Surgical excision of eutopic endometrial biopsy of patients with endometriosis and controls. MAIN OUTCOME MEASURE(S): Mitochondrial protein expression. RESULT(S): Seventy-eight qualified mitochondrial protein peaks were detected, ten of them had a significant difference. Three combined potential biomarkers, with mass-to-charge ratios (m/z) of 15,334, 15,128, and 16,069, were found, and the diagnostic system distinguished endometriosis from control samples with a specificity of 86.2% and a sensitivity of 87.5%. CONCLUSION(S): We discovered potential mitochondrial biomarkers of eutopic endometrium in endometriosis and set up a diagnostic model. Further identification of the proteins we found will help to explain pathology, new diagnoses, and therapeutic approaches for endometriosis. Crown
Authors: Devashana Gupta; M Louise Hull; Ian Fraser; Laura Miller; Patrick M M Bossuyt; Neil Johnson; Vicki Nisenblat Journal: Cochrane Database Syst Rev Date: 2016-04-20
Authors: Amelie Fassbender; Peter Simsa; Cleophas M Kyama; Etienne Waelkens; Attila Mihalyi; Christel Meuleman; Olivier Gevaert; Raf Van de Plas; Bart de Moor; Thomas M D'Hooghe Journal: Reprod Biol Endocrinol Date: 2010-10-21 Impact factor: 5.211