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Literature DB >> 20625766

Multilocus genetic analysis of Cryptosporidium parvum from Egypt.

Said Amer¹, Masoud Fayed, Hajime Honma, Yasuhiro Fukuda, Chica Tada, Yutaka Nakai.

Abstract

Cryptosporidium parvum is a ubiquitous zonootic parasite causing enteritis in man and animals. Cryptosporidium infection was confirmed microscopically in neonatal calves (less than 6 weeks of age) at Kafr El Sheikh Province, Egypt. Multilocus analysis using a wide array of genetic markers was carried out to assess genetic diversity of C. parvum isolates. PCR amplification and partial sequence analysis of 70 kDa heat shock protein, dihydrofolate reductase, alpha-tubulin, elongation factor 1 alpha as well as thrombospondin-related anonymous protein of Cryptosporidium-1, and thrombospondin-related anonymous protein of Cryptosporidium-2 gene markers were achieved. Data indicated that the analyzed isolates belong to C. parvum genotype II with obvious sequence heterogeneity compared with counterparts deposited in Genebank.

Entities: Disease Species

Mesh：

Substances：

Year: 2010 PMID： 20625766 DOI： 10.1007/s00436-010-1967-y

Source DB: PubMed Journal: Parasitol Res ISSN： 0932-0113 Impact factor: 2.289

39 in total

1. Prevalence of Giardia sp. Cryptosporidium parvum and Cryptosporidium andersoni (syn. C. muris) [correction of Cryptosporidium parvum and Cryptosporidium muris (C. andersoni)] in 109 dairy herds in five counties of southeastern New York.

Authors: S E Wade; H O Mohammed; S L Schaaf
Journal: Vet Parasitol Date: 2000-11-01 Impact factor: 2.738

2. Phylogenetic relationships of Cryptosporidium parasites based on the 70-kilodalton heat shock protein (HSP70) gene.

Authors: I M Sulaiman; U M Morgan; R C Thompson; A A Lal; L Xiao
Journal: Appl Environ Microbiol Date: 2000-06 Impact factor: 4.792

Review 3. 'The glideosome': a dynamic complex powering gliding motion and host cell invasion by Toxoplasma gondii.

Authors: Corinna Opitz; Dominique Soldati
Journal: Mol Microbiol Date: 2002-08 Impact factor: 3.501

4. CONFIDENCE LIMITS ON PHYLOGENIES: AN APPROACH USING THE BOOTSTRAP.

Authors: Joseph Felsenstein
Journal: Evolution Date: 1985-07 Impact factor: 3.694

5. The neighbor-joining method: a new method for reconstructing phylogenetic trees.

Authors: N Saitou; M Nei
Journal: Mol Biol Evol Date: 1987-07 Impact factor: 16.240

6. Cryptosporidium parvum infection involving novel genotypes in wildlife from lower New York State.

Authors: J F Perz; S M Le Blancq
Journal: Appl Environ Microbiol Date: 2001-03 Impact factor: 4.792

7. The thrombospondin-related protein CpMIC1 (CpTSP8) belongs to the repertoire of micronemal proteins of Cryptosporidium parvum.

Authors: Lorenza Putignani; Alessia Possenti; Simona Cherchi; Edoardo Pozio; Andrea Crisanti; Furio Spano
Journal: Mol Biochem Parasitol Date: 2007-09-29 Impact factor: 1.759

8. Potential antifolate resistance determinants and genotypic variation in the bifunctional dihydrofolate reductase-thymidylate synthase gene from human and bovine isolates of Cryptosporidium parvum.

Authors: J R Vásquez; L Goozé; K Kim; J Gut; C Petersen; R G Nelson
Journal: Mol Biochem Parasitol Date: 1996-08 Impact factor: 1.759