| Literature DB >> 20622442 |
Miyuki Muramatsu1, Junsuke Uwada, Naoyuki Matsumoto, Hisato Saitoh.
Abstract
We found that the small ubiquitin-related modifier (SUMO) conjugation reaction can be visualized simply by incubating green fluorescent protein (GFP)-SUMO-1 with permeabilized cells in the presence of ATP for 15 min. Neither special equipment for protein purification nor highly developed skills for recombinant technologies is required, making the assay potentially applicable to large-scale drug-screening strategies for the identification of drugs that can inhibit or enhance SUMOylation.Entities:
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Year: 2010 PMID： 20622442 DOI： 10.1271/bbb.100081
Source DB: PubMed Journal: Biosci Biotechnol Biochem ISSN： 0916-8451 Impact factor: 2.043