Literature DB >> 20621125

Development and inter-laboratory evaluation of real-time PCR assays for the detection of pospiviroids.

Wendy Monger1, Jenny Tomlinson, Neil Booonham, Mojca Virscek Marn, Irena Mavric Plesko, Valérie Molinero-Demilly, Xavier Tassus, Ellis Meekes, Marcel Toonen, Lambros Papayiannis, Zoila Perez-Egusquiza, Natasa Mehle, Claudia Jansen, Steen Lykke Nielsen.   

Abstract

Assays based on real-time PCR (TaqMan) that can detect a number of viroids in the genus Pospiviroid have been developed and evaluated. The assays are designed for detecting viroids from tomato leaf material but detection from other solanaceous hosts of these viroids has been confirmed. These methods have been validated by nine laboratories and comprise a reliable set of assays for the detection of CEVd, TASVd, CLVd and a generic assay which will detect the six viroids of concern to European tomato growers: PSTVd, TCDVd, CEVd, CLVd, TASVd and CSVd. Copyright (c) 2010. Published by Elsevier B.V.

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Year:  2010        PMID: 20621125     DOI: 10.1016/j.jviromet.2010.07.002

Source DB:  PubMed          Journal:  J Virol Methods        ISSN: 0166-0934            Impact factor:   2.014


  4 in total

1.  Survival and transmission of potato virus Y, pepino mosaic virus, and potato spindle tuber viroid in water.

Authors:  N Mehle; I Gutiérrez-Aguirre; N Prezelj; D Delic; U Vidic; M Ravnikar
Journal:  Appl Environ Microbiol       Date:  2013-12-13       Impact factor: 4.792

2.  Development of a multiplexed bead-based suspension array for the detection and discrimination of pospiviroid plant pathogens.

Authors:  Sharon L van Brunschot; Jan H W Bergervoet; Daniel E Pagendam; Marjanne de Weerdt; Andrew D W Geering; André Drenth; René A A van der Vlugt
Journal:  PLoS One       Date:  2014-01-03       Impact factor: 3.240

3.  A universal oligonucleotide microarray with a minimal number of probes for the detection and identification of viroids at the genus level.

Authors:  Yongjiang Zhang; Jun Yin; Dongmei Jiang; Yanyan Xin; Fang Ding; Ziniu Deng; Guoping Wang; Xianfeng Ma; Fang Li; Guifen Li; Mingfu Li; Shifang Li; Shuifang Zhu
Journal:  PLoS One       Date:  2013-05-29       Impact factor: 3.240

Review 4.  A current overview of two viroids that infect chrysanthemums: Chrysanthemum stunt viroid and Chrysanthemum chlorotic mottle viroid.

Authors:  Won Kyong Cho; Yeonhwa Jo; Kyoung-Min Jo; Kook-Hyung Kim
Journal:  Viruses       Date:  2013-04-17       Impact factor: 5.048

  4 in total

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