Y Shen1, Y Wang, D Li, C Wang, B Xu, G Dong, H Huang, H Jing. 1. Department of Cardio-thoracic Surgery, Jinling Hospital, Clinical Medicine School, Nanjing University, Nanjing, China.
Abstract
BACKGROUND: Ischemia-reperfusion (I/R) injury may influence graft function after transplantation. Erythropoietin (EPO) attenuates I/R injury in various animal organs such as intestine, brain, and kidney. OBJECTIVE: To evaluate the effects of pretreatment with recombinant human EPO (rhEPO) on I/R-induced heart injury. MATERIALS AND METHODS: A rat model of I/R injury was established by ligating the left descending coronary artery for 30 minutes, followed by reperfusion for 4 hours. Fifty Sprague-Dawley rats were divided into 5 groups: sham operation; I/R; I/R+rhEPO, 100 U/kg; I/R+rhEPO, 1000 U/kg; and I/R+rhEPO, 5000 U/kg. Electrocardiograms were assessed continuously to note arrhythmia caused by reperfusion. Serum concentrations of interleukin (IL)-6 and IL-8, and tumor necrosis factor-alpha were measured at 2 and 4 hours after reperfusion. RESULTS: The rhEPO-treated animals exhibited dosage-dependent significant reduction in the incidence of ventricular arrhythmia caused by reperfusion, and markedly decreased serum concentrations of IL-6, IL-8, and tumor necrosis factor-alpha (P < .05) compared with the I/R group (P < .05). CONCLUSION: The rhEPO attenuates myocardial I/R injury in rats, at least in part related to inhibition of the system inflammatory response.
BACKGROUND:Ischemia-reperfusion (I/R) injury may influence graft function after transplantation. Erythropoietin (EPO) attenuates I/R injury in various animal organs such as intestine, brain, and kidney. OBJECTIVE: To evaluate the effects of pretreatment with recombinant humanEPO (rhEPO) on I/R-induced heart injury. MATERIALS AND METHODS: A rat model of I/R injury was established by ligating the left descending coronary artery for 30 minutes, followed by reperfusion for 4 hours. Fifty Sprague-Dawley rats were divided into 5 groups: sham operation; I/R; I/R+rhEPO, 100 U/kg; I/R+rhEPO, 1000 U/kg; and I/R+rhEPO, 5000 U/kg. Electrocardiograms were assessed continuously to note arrhythmia caused by reperfusion. Serum concentrations of interleukin (IL)-6 and IL-8, and tumor necrosis factor-alpha were measured at 2 and 4 hours after reperfusion. RESULTS: The rhEPO-treated animals exhibited dosage-dependent significant reduction in the incidence of ventricular arrhythmia caused by reperfusion, and markedly decreased serum concentrations of IL-6, IL-8, and tumor necrosis factor-alpha (P < .05) compared with the I/R group (P < .05). CONCLUSION: The rhEPO attenuates myocardial I/R injury in rats, at least in part related to inhibition of the system inflammatory response.
Authors: Mirko Pesce; Paolo Felaco; Sara Franceschelli; Lorenza Speranza; Alfredo Grilli; Maria Anna De Lutiis; Alessio Ferrone; Vittorio Sirolli; Mario Bonomini; Mario Felaco; Antonia Patruno Journal: Open Biol Date: 2014-06 Impact factor: 6.411
Authors: Sophie de Seigneux; Belen Ponte; Lucien Weiss; Jérôme Pugin; Jacques André Romand; Pierre-Yves Martin; Patrick Saudan Journal: BMC Nephrol Date: 2012-10-03 Impact factor: 2.388