Literature DB >> 20619304

An improved 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) reduction assay for evaluating the viability of Escherichia coli cells.

Hengwei Wang1, Hairong Cheng, Fengqing Wang, Dongzhi Wei, Xuedong Wang.   

Abstract

To reduce the interference in MTT reduction assay, we developed an improved protocol for evaluating the viability of Escherichia coli cells by conducting MTT reduction in 1.5-mL centrifuge tubes and formazan dissolution in test tubes. Our study is helpful in developing protocols for measuring the viability of other gram-negative bacteria. (c) 2010 Elsevier B.V. All rights reserved.

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Year:  2010        PMID： 20619304     DOI： 10.1016/j.mimet.2010.06.014

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  48 in total

1.  SigB-dependent tolerance to protein synthesis-inhibiting antibiotics in Listeria monocytogenes EGDe.

Authors:  Qingchun Zhou; Li Wang; Xiaojiao Yin; Xiaoqin Feng; Junli Shang; Qin Luo
Journal:  Curr Microbiol       Date:  2011-12-04       Impact factor: 2.188

2.  Adaptation of Pseudomonas aeruginosa clinical isolates to benzalkonium chloride retards its growth and enhances biofilm production.

Authors:  Tarek El-Banna; Ahmed Abd El-Aziz; Fatma Sonbol; Engy El-Ekhnawy
Journal:  Mol Biol Rep       Date:  2019-04-11       Impact factor: 2.316

3.  Theobroma cacao: Review of the Extraction, Isolation, and Bioassay of Its Potential Anti-cancer Compounds.

Authors:  Zainal Baharum; Abdah Md Akim; Taufiq Yap Yun Hin; Roslida Abdul Hamid; Rosmin Kasran
Journal:  Trop Life Sci Res       Date:  2016-02

4.  The Proliferation Mechanism of Lactobacillus plantarum RB1 Stimulated by Stachyose.

Authors:  Qing Pan; Xiaoqun Zeng; Daodong Pan; Liuyang Peng; Zhen Wu; Yangying Sun; Yan Wei
Journal:  Curr Microbiol       Date:  2017-04-04       Impact factor: 2.188

5.  Relevance of FeoAB system in Rhodanobacter sp. B2A1Ga4 resistance to heavy metals, aluminium, gallium, and indium.

Authors:  Joana B Caldeira; Ana Paula Chung; Paula V Morais; Rita Branco
Journal:  Appl Microbiol Biotechnol       Date:  2021-04-01       Impact factor: 4.813

6.  Synthesis, Characterization of NR@SiO2/PNIPAm-co-Ppa Composite Nanogel and Study On Its Application in Photodynamic Therapy.

Authors:  Zirui Wang; Qiusheng Song; Lin Zhu; Chengyan Zhao; Haihong Ma
Journal:  J Fluoresc       Date:  2022-01-29       Impact factor: 2.217

7.  Impact of Membrane Phospholipid Alterations in Escherichia coli on Cellular Function and Bacterial Stress Adaptation.

Authors:  Veronica W Rowlett; Venkata K P S Mallampalli; Anja Karlstaedt; William Dowhan; Heinrich Taegtmeyer; William Margolin; Heidi Vitrac
Journal:  J Bacteriol       Date:  2017-06-13       Impact factor: 3.490

8.  Failsafe mechanisms couple division and DNA replication in bacteria.

Authors:  Heidi A Arjes; Allison Kriel; Nohemy A Sorto; Jared T Shaw; Jue D Wang; Petra Anne Levin
Journal:  Curr Biol       Date:  2014-08-28       Impact factor: 10.834

9.  Development of expanded host range phage active on biofilms of multi-drug resistant Pseudomonas aeruginosa.

Authors:  Abigail C Mapes; Barbara W Trautner; Kershena S Liao; Robert F Ramig
Journal:  Bacteriophage       Date:  2016-01-05

10.  Cytotoxicity and Microbicidal Activity of Commonly Used Organic Solvents: A Comparative Study and Application to a Standardized Extract from Vaccinium macrocarpon.

Authors:  Yana Ilieva; Lyudmila Dimitrova; Maya Margaritova Zaharieva; Mila Kaleva; Petko Alov; Ivanka Tsakovska; Tania Pencheva; Ivanka Pencheva-El Tibi; Hristo Najdenski; Ilza Pajeva
Journal:  Toxics       Date:  2021-04-21
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