Literature DB >> 2061686

Glass-bead column separation of motile and nonmotile human spermatozoa.

N Lechtzin1, W Garside, S Heyner, N Hillman.   

Abstract

Glass-bead columns were tested for their efficiency in concentrating motile human spermatozoa from frozen semen samples. The data show that glass-bead filtration concentrates the motile gametes in each sample and is significantly more efficient than the swim-up method for obtaining populations of motile spermatozoa. The data suggest that this method can be applied clinically to obtain motile spermatozoa from poor-quality semen for use in in vitro fertilization.

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Mesh：

Year:  1991        PMID： 2061686     DOI： 10.1007/bf01138662

Source DB:  PubMed          Journal:  J In Vitro Fert Embryo Transf        ISSN： 0740-7769


  14 in total

1.  Separation of dead and live mouse spermatozoa.

Authors:  J McGrath; N Hillman; M Nadijcka
Journal:  Dev Biol       Date:  1977-11       Impact factor: 3.582

2.  Are human spermatozoa separated on a Percoll density gradient safe for therapeutic use?

Authors:  S J Pickering; T P Fleming; P R Braude; V N Bolton; G A Gresham
Journal:  Fertil Steril       Date:  1989-06       Impact factor: 7.329

3.  A comparative study of buffer systems as cryoprotectants for human spermatozoa.

Authors:  G S Prins; L Weidel
Journal:  Fertil Steril       Date:  1986-07       Impact factor: 7.329

4.  Improvement in the quality and fertilization potential of a human sperm population using the rise technique.

Authors:  L D Russell; B J Rogers
Journal:  J Androl       Date:  1987 Jan-Feb

5.  The influence of seminal characteristics on the success rate of human in vitro fertilization.

Authors:  M M Mahadevan; A O Trounson
Journal:  Fertil Steril       Date:  1984-09       Impact factor: 7.329

6.  Improved sperm concentration, motility, and fertilization rates following Ficoll treatment of sperm in a human in vitro fertilization program.

Authors:  A Bongso; S C Ng; H Mok; M N Lim; H L Teo; P C Wong; S Ratnam
Journal:  Fertil Steril       Date:  1989-05       Impact factor: 7.329

7.  A rapid method for the fractionation of human spermatozoa according to their progressive motility.

Authors:  F K Gorus; D G Pipeleers
Journal:  Fertil Steril       Date:  1981-06       Impact factor: 7.329

8.  Effect of sperm motility on human in vitro fertilization.

Authors:  T A Bongso; S C Ng; H Mok; M N Lim; H L Teo; P C Wong; S S Ratnam
Journal:  Arch Androl       Date:  1989

9.  Correlation between in vitro fertilization and human sperm density and motility.

Authors:  J Gerris; I Khan
Journal:  J Androl       Date:  1987 Jan-Feb

10.  Cryosurvival of human spermatozoa frozen in eight different buffer systems.

Authors:  L Weidel; G S Prins
Journal:  J Androl       Date:  1987 Jan-Feb
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