Literature DB >> 20616806

Structural insight into the oxidation-sensing mechanism of the antibiotic resistance of regulator MexR.

Hao Chen1, Chengqi Yi, Jin Zhang, Wenru Zhang, Zhiyun Ge, Cai-Guang Yang, Chuan He.   

Abstract

MexR functions as the primary regulator of the mexAB-oprM multidrug efflux expression in Pseudomonas aeruginosa. It has been shown that MexR senses oxidative stress by interprotomer disulphide bond formation between redox-active cysteines. This oxidation induces MexR to dissociate from the promoter DNA, thus activating the transcriptional expression of efflux pump genes. In this study, we present the crystal structure of MexR in its oxidized form at a resolution of 2.1 A. This crystal structure reveals the mechanism by which oxidative signal allosterically derepresses the MexR-controlled transcription activation.

Entities:  

Mesh：

Substances：

Year:  2010        PMID： 20616806      PMCID： PMC2933878          DOI： 10.1038/embor.2010.96

Source DB:  PubMed          Journal:  EMBO Rep        ISSN： 1469-221X            Impact factor:   8.807


  18 in total

1.  nalD encodes a second repressor of the mexAB-oprM multidrug efflux operon of Pseudomonas aeruginosa.

Authors:  Yuji Morita; Lily Cao; Virginia C Gould; Matthew B Avison; Keith Poole
Journal:  J Bacteriol       Date:  2006-10-06       Impact factor: 3.490

Review 2.  Discovering mechanisms of signaling-mediated cysteine oxidation.

Authors:  Leslie B Poole; Kimberly J Nelson
Journal:  Curr Opin Chem Biol       Date:  2008-03-07       Impact factor: 8.822

Review 3.  Structure, function and inhibition of RND efflux pumps in Gram-negative bacteria: an update.

Authors:  Jessica M A Blair; Laura J V Piddock
Journal:  Curr Opin Microbiol       Date:  2009-08-05       Impact factor: 7.934

4.  Structural basis of the redox switch in the OxyR transcription factor.

Authors:  H Choi; S Kim; P Mukhopadhyay; S Cho; J Woo; G Storz; S E Ryu
Journal:  Cell       Date:  2001-04-06       Impact factor: 41.582

5.  An oxidation-sensing mechanism is used by the global regulator MgrA in Staphylococcus aureus.

Authors:  Peng R Chen; Taeok Bae; Wade A Williams; Erica M Duguid; Phoebe A Rice; Olaf Schneewind; Chuan He
Journal:  Nat Chem Biol       Date:  2006-09-17       Impact factor: 15.040

6.  The crystal structure of MexR from Pseudomonas aeruginosa in complex with its antirepressor ArmR.

Authors:  Mark S Wilke; Markus Heller; A Louise Creagh; Charles A Haynes; Lawrence P McIntosh; Keith Poole; Natalie C J Strynadka
Journal:  Proc Natl Acad Sci U S A       Date:  2008-09-23       Impact factor: 11.205

7.  Pseudomonas aeruginosa OspR is an oxidative stress sensing regulator that affects pigment production, antibiotic resistance and dissemination during infection.

Authors:  Lefu Lan; Thomas S Murray; Barbara I Kazmierczak; Chuan He
Journal:  Mol Microbiol       Date:  2009-11-25       Impact factor: 3.501

8.  A common mechanism of cellular death induced by bactericidal antibiotics.

Authors:  Michael A Kohanski; Daniel J Dwyer; Boris Hayete; Carolyn A Lawrence; James J Collins
Journal:  Cell       Date:  2007-09-07       Impact factor: 41.582

9.  Structural mechanism of organic hydroperoxide induction of the transcription regulator OhrR.

Authors:  Kate J Newberry; Mayuree Fuangthong; Warunya Panmanee; Skorn Mongkolsuk; Richard G Brennan
Journal:  Mol Cell       Date:  2007-11-30       Impact factor: 17.970

10.  The Pseudomonas aeruginosa multidrug efflux regulator MexR uses an oxidation-sensing mechanism.

Authors:  Hao Chen; Jie Hu; Peng R Chen; Lefu Lan; Zigang Li; Leslie M Hicks; Aaron R Dinner; Chuan He
Journal:  Proc Natl Acad Sci U S A       Date:  2008-08-29       Impact factor: 11.205
View more
  18 in total

1.  A dual role of the transcriptional regulator TstR provides insights into cyanide detoxification in Lactobacillus brevis.

Authors:  Fernando A Pagliai; Caitlin C Murdoch; Sara M Brown; Claudio F Gonzalez; Graciela L Lorca
Journal:  Mol Microbiol       Date:  2014-04-14       Impact factor: 3.501

2.  Quorum quenching quandary: resistance to antivirulence compounds.

Authors:  Toshinari Maeda; Rodolfo García-Contreras; Mingming Pu; Lili Sheng; Luis Rene Garcia; Maria Tomás; Thomas K Wood
Journal:  ISME J       Date:  2011-09-15       Impact factor: 10.302

Review 3.  The challenge of efflux-mediated antibiotic resistance in Gram-negative bacteria.

Authors:  Xian-Zhi Li; Patrick Plésiat; Hiroshi Nikaido
Journal:  Clin Microbiol Rev       Date:  2015-04       Impact factor: 26.132

4.  Pseudomonas aeruginosa thiol peroxidase protects against hydrogen peroxide toxicity and displays atypical patterns of gene regulation.

Authors:  Nawarat Somprasong; Thichakorn Jittawuttipoka; Jintana Duang-Nkern; Adisak Romsang; Pimchai Chaiyen; Herbert P Schweizer; Paiboon Vattanaviboon; Skorn Mongkolsuk
Journal:  J Bacteriol       Date:  2012-05-18       Impact factor: 3.490

Review 5.  MarR family proteins are important regulators of clinically relevant antibiotic resistance.

Authors:  Grace A Beggs; Richard G Brennan; Mehreen Arshad
Journal:  Protein Sci       Date:  2019-11-14       Impact factor: 6.725

6.  Oxidation-sensing regulator AbfR regulates oxidative stress responses, bacterial aggregation, and biofilm formation in Staphylococcus epidermidis.

Authors:  Xing Liu; Xiaoxu Sun; Youcong Wu; Cen Xie; Wenru Zhang; Dan Wang; Xiaoyan Chen; Di Qu; Jianhua Gan; Hao Chen; Hualiang Jiang; Lefu Lan; Cai-Guang Yang
Journal:  J Biol Chem       Date:  2012-12-27       Impact factor: 5.157

7.  EmrA1 membrane fusion protein of Francisella tularensis LVS is required for resistance to oxidative stress, intramacrophage survival and virulence in mice.

Authors:  Zhuo Ma; Sukalyani Banik; Harshita Rane; Vanessa T Mora; Seham M Rabadi; Christopher R Doyle; David G Thanassi; Chandra Shekhar Bakshi; Meenakshi Malik
Journal:  Mol Microbiol       Date:  2014-02-08       Impact factor: 3.501

8.  Two distinct mechanisms of transcriptional regulation by the redox sensor YodB.

Authors:  Sang Jae Lee; In-Gyun Lee; Ki-Young Lee; Dong-Gyun Kim; Hyun-Jong Eun; Hye-Jin Yoon; Susanna Chae; Sung-Hyun Song; Sa-Ouk Kang; Min-Duk Seo; Hyoun Sook Kim; Sung Jean Park; Bong-Jin Lee
Journal:  Proc Natl Acad Sci U S A       Date:  2016-08-16       Impact factor: 11.205

9.  Crystal structures of SlyA protein, a master virulence regulator of Salmonella, in free and DNA-bound states.

Authors:  Kyle T Dolan; Erica M Duguid; Chuan He
Journal:  J Biol Chem       Date:  2011-05-05       Impact factor: 5.157

Review 10.  Redox active thiol sensors of oxidative and nitrosative stress.

Authors:  Andrés Vázquez-Torres
Journal:  Antioxid Redox Signal       Date:  2012-03-15       Impact factor: 8.401
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.