Literature DB >> 2061615

ELISA procedures for the measurement of IgG subclass antibodies to bacterial antigens.

S Ruths1, P C Driedijk, R S Weening, T A Out.   

Abstract

We have developed enzyme-linked immunosorbent assays (ELISA) of IgG subclass antibodies against whole bacteria and bacterial antigens using enzyme-labelled mouse monoclonal antibodies. The properties of different anti-subclass antibodies were compared. In sera from 18 healthy adults we measured the IgG subclass distribution of specific antibodies against Staphylococcus aureus and Haemophilus influenzae b and against distinct bacterial components: pneumococcal capsular polysaccharides, dextran and tetanus toxoid. We found that antibodies against protein (tetanus toxoid) were mainly IgG1, with some contribution of IgG4 and IgG2. Antibodies against polysaccharides (pneumococcal PS and dextran) and whole bacteria were restricted mainly to IgG1 and IgG2.

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Year:  1991        PMID： 2061615     DOI： 10.1016/0022-1759(91)90127-2

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  5 in total

1.  Role of neutrophil Fc gamma RIIa (CD32) and Fc gamma RIIIb (CD16) polymorphic forms in phagocytosis of human IgG1- and IgG3-opsonized bacteria and erythrocytes.

Authors:  R G Bredius; C A Fijen; M De Haas; E J Kuijper; R S Weening; J G Van de Winkel; T A Out
Journal:  Immunology       Date:  1994-12       Impact factor: 7.397

2.  Simultaneous identification of antibodies to Brucella abortus and Staphylococcus aureus in milk samples by flow cytometry.

Authors:  D Iannelli; L D'Apice; D Fenizia; L Serpe; C Cottone; M Viscardi; R Capparelli
Journal:  J Clin Microbiol       Date:  1998-03       Impact factor: 5.948

3.  Complement activation by polyclonal immunoglobulin G1 and G2 antibodies against Staphylococcus aureus, Haemophilus influenzae type b, and tetanus toxoid.

Authors:  R G Bredius; P C Driedijk; M F Schouten; R S Weening; T A Out
Journal:  Infect Immun       Date:  1992-11       Impact factor: 3.441

4.  CD20 deficiency in humans results in impaired T cell-independent antibody responses.

Authors:  Taco W Kuijpers; Richard J Bende; Paul A Baars; Annette Grummels; Ingrid A M Derks; Koert M Dolman; Tim Beaumont; Thomas F Tedder; Carel J M van Noesel; Eric Eldering; René A W van Lier
Journal:  J Clin Invest       Date:  2009-12-21       Impact factor: 14.808

5.  Neonatal infections in Saudi Arabia: association with C-reactive protein, CRP -286 (C>T>A) gene polymorphism and IgG antibodies.

Authors:  Amre Nasr; Gamal Allam; Ali Al-Zahrani; Adnan Alsulaimani
Journal:  BMC Immunol       Date:  2013-08-13       Impact factor: 3.615
  5 in total

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