| Literature DB >> 2060648 |
Y L Khmelnitsky1, A B Belova, A V Levashov, V V Mozhaev.
Abstract
The stability of alpha-chymotrypsin covalently modified with a strongly hydrophilic modifier, pyromellitic dianhydride, against solvent-induced denaturation in water-organic solvent binary mixtures has been studied. It was found that the hydrophilization results in a strong stabilization of the enzyme against denaturation by organic solvents. The stabilizing effect is explained in terms of the enhanced ability of the hydrophilized enzyme to keep its hydration shell, which is indispensable for supporting the native protein conformation, from denaturing stripping by organic solvents.Entities:
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Year: 1991 PMID： 2060648 DOI： 10.1016/0014-5793(91)80700-d
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124