Literature DB >> 20606292

Characterization, crystallization and preliminary X-ray analysis of the adhesive domain of SdrE from Staphylococcus aureus.

Hua Xiang1, Fangfang Gao, Dacheng Wang, Jing Liu, Jia Hu, Liqing Zhang, Shentao Li, Xuming Deng.   

Abstract

The adhesive domain of SdrE from Staphylococcus aureus was recombinantly expressed in Escherichia coli. The purified protein was identified by SDS-PAGE and MALDI-TOF MS. The protein was crystallized using the vapour-diffusion method in hanging-drop mode with PEG 8000 as the primary precipitating agent. X-ray diffraction data were collected to 1.8 A resolution from a single crystal of the protein. Preliminary X-ray analysis indicated that the crystal belonged to space group P1, with unit-cell parameters a = 40.714, b = 66.355, c = 80.827 A, alpha = 111.19, beta = 93.99, gamma = 104.39 degrees.

Entities:  

Mesh：

Substances：

Year:  2010        PMID： 20606292      PMCID： PMC2898480          DOI： 10.1107/S1744309110020907

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  15 in total

1.  Survey of infections due to Staphylococcus species: frequency of occurrence and antimicrobial susceptibility of isolates collected in the United States, Canada, Latin America, Europe, and the Western Pacific region for the SENTRY Antimicrobial Surveillance Program, 1997-1999.

Authors:  D J Diekema; M A Pfaller; F J Schmitz; J Smayevsky; J Bell; R N Jones; M Beach
Journal:  Clin Infect Dis       Date:  2001-05-15       Impact factor: 9.079

2.  A novel variant of the immunoglobulin fold in surface adhesins of Staphylococcus aureus: crystal structure of the fibrinogen-binding MSCRAMM, clumping factor A.

Authors:  Champion C S Deivanayagam; Elisabeth R Wann; Wei Chen; Mike Carson; Kanagalaghatta R Rajashankar; Magnus Höök; Sthanam V L Narayana
Journal:  EMBO J       Date:  2002-12-16       Impact factor: 11.598

Review 3.  MSCRAMM-mediated adherence of microorganisms to host tissues.

Authors:  J M Patti; B L Allen; M J McGavin; M Höök
Journal:  Annu Rev Microbiol       Date:  1994       Impact factor: 15.500

4.  Structural organization of the fibrinogen-binding region of the clumping factor B MSCRAMM of Staphylococcus aureus.

Authors:  S Perkins; E J Walsh; C C Deivanayagam; S V Narayana; T J Foster; M Höök
Journal:  J Biol Chem       Date:  2001-09-21       Impact factor: 5.157

5.  Staphylococcus aureus clumping factor B (ClfB) promotes adherence to human type I cytokeratin 10: implications for nasal colonization.

Authors:  Louise M O'Brien; Evelyn J Walsh; Ruth C Massey; Sharon J Peacock; Timothy J Foster
Journal:  Cell Microbiol       Date:  2002-11       Impact factor: 3.715

6.  Identification of residues in the Staphylococcus aureus fibrinogen-binding MSCRAMM clumping factor A (ClfA) that are important for ligand binding.

Authors:  O M Hartford; E R Wann; M Höök; T J Foster
Journal:  J Biol Chem       Date:  2000-10-23       Impact factor: 5.157

7.  Multiple mechanisms for the activation of human platelet aggregation by Staphylococcus aureus: roles for the clumping factors ClfA and ClfB, the serine-aspartate repeat protein SdrE and protein A.

Authors:  Louise O'Brien; Steven W Kerrigan; Gideon Kaw; Michael Hogan; José Penadés; David Litt; Desmond J Fitzgerald; Timothy J Foster; Dermot Cox
Journal:  Mol Microbiol       Date:  2002-05       Impact factor: 3.501

8.  A "dock, lock, and latch" structural model for a staphylococcal adhesin binding to fibrinogen.

Authors:  Karthe Ponnuraj; M Gabriela Bowden; Stacey Davis; S Gurusiddappa; Dwight Moore; Damon Choe; Yi Xu; Magnus Hook; Sthanam V L Narayana
Journal:  Cell       Date:  2003-10-17       Impact factor: 41.582

Review 9.  Staphylococcus aureus: superbug, super genome?

Authors:  Jodi A Lindsay; Matthew T G Holden
Journal:  Trends Microbiol       Date:  2004-08       Impact factor: 17.079

Review 10.  Regulation of virulence determinants in vitro and in vivo in Staphylococcus aureus.

Authors:  Ambrose L Cheung; Arnold S Bayer; Gongyi Zhang; Hattie Gresham; Yan-Qiong Xiong
Journal:  FEMS Immunol Med Microbiol       Date:  2004-01-15
View more
  1 in total

1.  Comparative Transcriptomic Analysis of Staphylococcus aureus Associated with Periprosthetic Joint Infection under in Vivo and in Vitro Conditions.

Authors:  Thao Le Masters; Stephen Johnson; Patricio R Jeraldo; Kerryl E Greenwood-Quaintance; Scott A Cunningham; Matthew P Abdel; Nicholas Chia; Robin Patel
Journal:  J Mol Diagn       Date:  2021-06-05       Impact factor: 5.341
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.