Literature DB >> 20606279

Crystallization and preliminary diffraction analysis of an engineered cephalosporin acylase.

Anandhi Anandan1, Corinne Vallet, Travis Coyle, Ibrahim M Moustafa, Alice Vrielink.   

Abstract

Crystallization conditions are reported for an engineered cephalosporin acylase based on the sequence of glutaryl-7-aminocephalosporanic acid acylase from Pseudomonas strain N176. Initial crystals were grown using polyethylene glycol as a crystallizing agent; however, these crystals diffracted poorly and exhibited high mosaicity. A dehydration procedure in which crystals were transferred to a solution containing a higher concentration of polyethylene glycol as well as glycerol improved the diffraction quality such that a 1.57 A diffraction data set could be obtained.

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Year:  2010        PMID： 20606279      PMCID： PMC2898467          DOI： 10.1107/S1744309110017185

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  6 in total

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Authors: 
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1994-09-01

2.  Cloning and characterization of the genes for two distinct cephalosporin acylases from a Pseudomonas strain.

Authors:  A Matsuda; K Matsuyama; K Yamamoto; S Ichikawa; K Komatsu
Journal:  J Bacteriol       Date:  1987-12       Impact factor: 3.490

3.  In vivo post-translational processing and subunit reconstitution of cephalosporin acylase from Pseudomonas sp. 130.

Authors:  Y Li; J Chen; W Jiang; X Mao; G Zhao; E Wang
Journal:  Eur J Biochem       Date:  1999-06

4.  The 2.0 A crystal structure of cephalosporin acylase.

Authors:  Y Kim; K Yoon; Y Khang; S Turley; W G Hol
Journal:  Structure       Date:  2000-10-15       Impact factor: 5.006

5.  Evolution of an acylase active on cephalosporin C.

Authors:  Loredano Pollegioni; Simona Lorenzi; Elena Rosini; Giorgia Letizia Marcone; Gianluca Molla; Roberto Verga; Walter Cabri; Mirella S Pilone
Journal:  Protein Sci       Date:  2005-10-31       Impact factor: 6.725

6.  Deacylation activity of cephalosporin acylase to cephalosporin C is improved by changing the side-chain conformations of active-site residues.

Authors:  Bora Oh; Myungsook Kim; Jongchul Yoon; Kyungwha Chung; Yongchul Shin; Dongsoon Lee; Youngsoo Kim
Journal:  Biochem Biophys Res Commun       Date:  2003-10-10       Impact factor: 3.575
  6 in total
  1 in total

1.  Increasing the X-ray diffraction power of protein crystals by dehydration: the case of bovine serum albumin and a survey of literature data.

Authors:  Irene Russo Krauss; Filomena Sica; Carlo Andrea Mattia; Antonello Merlino
Journal:  Int J Mol Sci       Date:  2012-03-21       Impact factor: 6.208
  1 in total

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