A sensitive choline biosensor with supramolecular architecture.

A sensitive biosensor with supramolecular architecture was designed and implemented here to detect choline. Choline oxidase and horseradish peroxidase were assembled onto the polymer of thiolated beta-cyclodextrin and platinum nanoparticles modified gold electrode through 1-adamantane carboxylic acid coupling. Square wave voltammetry showed that the reduction currents at 0.38 mV had a linear relationship with the logarithms of choline concentrations in the range of 10(-9)-10(-2) M, and the detection limit was down to 0.1 nM. Such biosensor also exhibited excellent selectivity, reproducibility and stability. Copyright 2010 Elsevier B.V. All rights reserved.