Literature DB >> 20600092

Multiplex RT-PCR with broad-range primers and an exogenous internal amplification control for the detection of honeybee viruses in bumblebees.

Ivan Meeus1, Guy Smagghe, Reinhold Siede, Kris Jans, Dirk C de Graaf.   

Abstract

Bumblebees are commercially reared and transported worldwide mainly for pollination of greenhouse tomatoes. Three honeybee viruses have been reported in bumblebees: Acute bee paralysis virus, Kashmir bee virus and Deformed wing virus. We developed a multiplex RT-PCR with primers designed on highly conserved regions of the RNA-dependent RNA polymerase in order to detect a maximum range of viral variants. Rearing facilities and governmental organizations can now thoroughly screen bumblebee colonies with a cost-effective technique with an integrated internal amplification control (IAC) implementable in laboratories that strive for International Organization for Standardization (ISO) certification. Copyright 2010 Elsevier Inc. All rights reserved.

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Year:  2010        PMID： 20600092     DOI： 10.1016/j.jip.2010.06.012

Source DB:  PubMed          Journal:  J Invertebr Pathol        ISSN： 0022-2011            Impact factor:   2.841


  8 in total

Review 1.  Molecular Detection and Differentiation of Arthropod, Fungal, Protozoan, Bacterial and Viral Pathogens of Honeybees.

Authors:  Lucas Lannutti; Fernanda Noemi Gonzales; Maria José Dus Santos; Mónica Florin-Christensen; Leonhard Schnittger
Journal:  Vet Sci       Date:  2022-05-02

2.  BeeDoctor, a versatile MLPA-based diagnostic tool for screening bee viruses.

Authors:  Lina De Smet; Jorgen Ravoet; Joachim R de Miranda; Tom Wenseleers; Matthias Y Mueller; Robin F A Moritz; Dirk C de Graaf
Journal:  PLoS One       Date:  2012-10-29       Impact factor: 3.240

3.  RNA viruses in hymenopteran pollinators: evidence of inter-Taxa virus transmission via pollen and potential impact on non-Apis hymenopteran species.

Authors:  Rajwinder Singh; Abby L Levitt; Edwin G Rajotte; Edward C Holmes; Nancy Ostiguy; Dennis Vanengelsdorp; W Ian Lipkin; Claude W Depamphilis; Amy L Toth; Diana L Cox-Foster
Journal:  PLoS One       Date:  2010-12-22       Impact factor: 3.240

4.  Sequence recombination and conservation of Varroa destructor virus-1 and deformed wing virus in field collected honey bees (Apis mellifera).

Authors:  Hui Wang; Jiazheng Xie; Tim G Shreeve; Jinmin Ma; Denise W Pallett; Linda A King; Robert D Possee
Journal:  PLoS One       Date:  2013-09-18       Impact factor: 3.240

5.  The relationship between managed bees and the prevalence of parasites in bumblebees.

Authors:  Peter Graystock; Dave Goulson; William O H Hughes
Journal:  PeerJ       Date:  2014-08-12       Impact factor: 2.984

Review 6.  Current Status of Loop-Mediated Isothermal Amplification Technologies for the Detection of Honey Bee Pathogens.

Authors:  Timothy C Cameron; Danielle Wiles; Travis Beddoe
Journal:  Front Vet Sci       Date:  2021-04-12

7.  Assessing the toxicological interaction effects of imidacloprid, thiamethoxam, and chlorpyrifos on Bombus terrestris based on the combination index.

Authors:  Yongkui Zhang; Dongqiang Zeng; Lu Li; Xiuchun Hong; Hongmei Li-Byarlay; Shudong Luo
Journal:  Sci Rep       Date:  2022-04-15       Impact factor: 4.996

8.  Pathogen prevalence in commercially reared bumble bees and evidence of spillover in conspecific populations.

Authors:  Tomás E Murray; Mary F Coffey; Eamonn Kehoe; Finbarr G Horgan
Journal:  Biol Conserv       Date:  2013-01-21       Impact factor: 5.990
  8 in total

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