Literature DB >> 20588906

Measurement of the ratio of forward-propagating to back-propagating second harmonic signal using a single objective.

Xiaoxing Han1, Edward Brown.   

Abstract

In this paper, we present a method to determine, for the first time, the ratio of forward-propagating second harmonic (SHG) signal to back-propagating SHG signal (F/B) in vivo on the surface of intact tissue samples without any biopsy or tissue sectioning, using only epidetection (i.e., via a single objective lens). This method has the additional benefit of using the confocal detection apparatus already contained within common commercially available two-photon laser-scanning microscopes, and hence can allow the measurement of the SHG F/B ratio in vivo with minimal purchase of new equipment. (c) 2010 Optical Society of America.

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Mesh：

Year:  2010        PMID： 20588906      PMCID： PMC3427947          DOI： 10.1364/OE.18.010538

Source DB:  PubMed          Journal:  Opt Express        ISSN： 1094-4087            Impact factor:   3.894


  11 in total

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9.  Alterations of the extracellular matrix in ovarian cancer studied by Second Harmonic Generation imaging microscopy.

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  6 in total

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5.  Structural changes in mixed Col I/Col V collagen gels probed by SHG microscopy: implications for probing stromal alterations in human breast cancer.

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  6 in total

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