OBJECTIVE: Trefoil factor (TFF) peptides are expressed in gastric tissues, where they are part of the epithelial defences. To complement previous in vitro work, the goal of the present study was to examine directly if TFF2 was essential for gastric restitution in vivo during the recovery from microscopic damage. DESIGN: TFF2 mutant (KO) mice were examined to study the epithelial repair process in vivo after laser-induced photodamage (LPD). Using two-photon laser energy absorption (710 nm), LPD was imposed on an approximately 3-5 cell region of surface epithelium in anaesthetised mouse stomach. Responses to damage were evaluated during confocal time-lapse microscopy; including area of damage and the extracellular pH adjacent to the damaged surface (Cl-NERF pH sensor). RESULTS: In control (TFF2+/+ and TFF2+/-) mice, damaged cells were exfoliated and the damaged epithelium was repaired by indomethacin. The resting surface pH was similar between control and TFF2-KO animals, but the post-LPD alkalisation of surface pH observed in control mice (pH 0.3 + or - 0.05, n=21) was attenuated in the TFF2-KO stomach (pH -0.08 + or - 0.09, n=18). Recobinant rat TFF3 partially rescued the attenuated surface pH change in TFF2-KO stomach, in the presence or absence of indomethacin. CONCLUSIONS: In the gastric epithelium in vivo, TFFs promote epithelial restitution via a mechanism that does not require cyclooxygenase activation. A novel role for TFFs to affect gastric surface pH is observed.
OBJECTIVE: Trefoil factor (TFF) peptides are expressed in gastric tissues, where they are part of the epithelial defences. To complement previous in vitro work, the goal of the present study was to examine directly if TFF2 was essential for gastric restitution in vivo during the recovery from microscopic damage. DESIGN:TFF2 mutant (KO) mice were examined to study the epithelial repair process in vivo after laser-induced photodamage (LPD). Using two-photon laser energy absorption (710 nm), LPD was imposed on an approximately 3-5 cell region of surface epithelium in anaesthetised mouse stomach. Responses to damage were evaluated during confocal time-lapse microscopy; including area of damage and the extracellular pH adjacent to the damaged surface (Cl-NERF pH sensor). RESULTS: In control (TFF2+/+ and TFF2+/-) mice, damaged cells were exfoliated and the damaged epithelium was repaired by indomethacin. The resting surface pH was similar between control and TFF2-KO animals, but the post-LPD alkalisation of surface pH observed in control mice (pH 0.3 + or - 0.05, n=21) was attenuated in the TFF2-KO stomach (pH -0.08 + or - 0.09, n=18). Recobinant ratTFF3 partially rescued the attenuated surface pH change in TFF2-KO stomach, in the presence or absence of indomethacin. CONCLUSIONS: In the gastric epithelium in vivo, TFFs promote epithelial restitution via a mechanism that does not require cyclooxygenase activation. A novel role for TFFs to affect gastric surface pH is observed.
Authors: S Rodrigues; Q D Nguyen; S Faivre; E Bruyneel; L Thim; B Westley; F May; G Flatau; M Mareel; C Gespach; S Emami Journal: FASEB J Date: 2001-07 Impact factor: 5.191
Authors: Eitaro Aihara; Courtney L Hentz; Abraham M Korman; Nicholas P J Perry; Vikram Prasad; Gary E Shull; Marshall H Montrose Journal: J Biol Chem Date: 2013-10-11 Impact factor: 5.157
Authors: Kristen A Engevik; Hikaru Hanyu; Andrea L Matthis; Tongli Zhang; Mark R Frey; Yusuke Oshima; Eitaro Aihara; Marshall H Montrose Journal: J Physiol Date: 2019-04-14 Impact factor: 5.182
Authors: Eitaro Aihara; Neisha M Medina-Candelaria; Hikaru Hanyu; Andrea L Matthis; Kristen A Engevik; Christine B Gurniak; Walter Witke; Jerrold R Turner; Tongli Zhang; Marshall H Montrose Journal: J Cell Sci Date: 2018-08-20 Impact factor: 5.285