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Literature DB >> 20581829

Simultaneous intracellular chloride and pH measurements using a GFP-based sensor.

Daniele Arosio¹, Fernanda Ricci, Laura Marchetti, Roberta Gualdani, Lorenzo Albertazzi, Fabio Beltram.

Abstract

Chloride and protons perform important closely related roles in many cellular responses. Here we developed a ratiometric biosensor, ClopHensor, based on a highly chloride-sensitive Aequorea victoria GFP variant that is suited for the combined real-time optical detection of pH changes and chloride fluxes in live cells. We detected high chloride concentration in large dense-core exocytosis granules by targeting ClopHensor to these intracellular compartments.

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Year: 2010 PMID： 20581829 DOI： 10.1038/nmeth.1471

Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547

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