BACKGROUND: This study tested whether rats with obesity induced by a hypercaloric diet (HD) present higher nutritional, endocrine, and cardiovascular disturbances compared with counterparts with obesity induced by overfeeding of a standard diet. An additional objective was to compare the isolated influence of HD on these parameters in lean and obese rats. MATERIAL/ METHODS: Twenty Wistar-Kyoto rats were distributed into four groups: CD-lean, CD-obese, HD-lean, and HD-obese. CD (control diet) and HD groups received commercial standard chow and HD, respectively, for 20 weeks. The lean and obese groups included obesity-resistant and obesity-prone animals, respectively. Nutritional and metabolic evaluation involved measurement of calorie intake, dietary efficiency, body weight, adiposity, glycemia, triacylglycerol, insulin, and leptin. Cardiovascular evaluation included systolic blood pressure measurement, echocardiography, and analyses of myocardial morphology and myosin heavy-chain composition. RESULTS: In both diets, obesity was characterized by increased adiposity, hyperleptinemia, hypertriacylglycerolemia, hyperinsulinemia, and cardiomyocyte nuclear hypertrophy. HD promoted hyperleptinemia and cardiac remodeling, characterized by nuclear and ventricular hypertrophy, as well as improved systolic performance in both the obesity-prone and obesity-resistant biotypes. In contrast to HD-lean, HD-obese rats presented more accentuated endocrine responses, including hyperglycemia, lower glycemic tolerance, and hyperleptinemia as well as interstitial fibrosis compared with the CD-obese animals. CONCLUSIONS: This study confirmed the primary hypothesis that rats with HD-induced obesity present more accentuated nutritional and endocrine disturbances compared with their counterparts with obesity resulting from overfeeding. In addition, dietary effects were more important between the obese groups, supporting evidence of an interaction between diet and biotype.
BACKGROUND: This study tested whether rats with obesity induced by a hypercaloric diet (HD) present higher nutritional, endocrine, and cardiovascular disturbances compared with counterparts with obesity induced by overfeeding of a standard diet. An additional objective was to compare the isolated influence of HD on these parameters in lean and obeserats. MATERIAL/ METHODS: Twenty Wistar-Kyoto rats were distributed into four groups: CD-lean, CD-obese, HD-lean, and HD-obese. CD (control diet) and HD groups received commercial standard chow and HD, respectively, for 20 weeks. The lean and obese groups included obesity-resistant and obesity-prone animals, respectively. Nutritional and metabolic evaluation involved measurement of calorie intake, dietary efficiency, body weight, adiposity, glycemia, triacylglycerol, insulin, and leptin. Cardiovascular evaluation included systolic blood pressure measurement, echocardiography, and analyses of myocardial morphology and myosin heavy-chain composition. RESULTS: In both diets, obesity was characterized by increased adiposity, hyperleptinemia, hypertriacylglycerolemia, hyperinsulinemia, and cardiomyocyte nuclear hypertrophy. HD promoted hyperleptinemia and cardiac remodeling, characterized by nuclear and ventricular hypertrophy, as well as improved systolic performance in both the obesity-prone and obesity-resistant biotypes. In contrast to HD-lean, HD-obeserats presented more accentuated endocrine responses, including hyperglycemia, lower glycemic tolerance, and hyperleptinemia as well as interstitial fibrosis compared with the CD-obese animals. CONCLUSIONS: This study confirmed the primary hypothesis that rats with HD-induced obesity present more accentuated nutritional and endocrine disturbances compared with their counterparts with obesity resulting from overfeeding. In addition, dietary effects were more important between the obese groups, supporting evidence of an interaction between diet and biotype.
Authors: Elaine Farah; Ana Lucia Cogni; Marcos F Minicucci; Paula S Azevedo; Katashi Okoshi; Beatriz B Matsubara; Silméia G Zanati; Rodrigo Haggeman; Sergio A R Paiva; Leonardo A M Zornoff Journal: Med Sci Monit Date: 2012-05
Authors: Paula S Azevedo; Bertha F Polegato; Marcos F Minicucci; Stephan M Pio; Igor A Silva; Priscila P Santos; Katashi Okoshi; Sergio A R Paiva; Leonardo A M Zornoff Journal: Med Sci Monit Date: 2012-07
Authors: Silvio A Oliveira Junior; Carlos R Padovani; Sergio A Rodrigues; Nilza R Silva; Paula F Martinez; Dijon Hs Campos; Marina P Okoshi; Katashi Okoshi; Maeli Dal-Pai; Antonio C Cicogna Journal: Cardiovasc Diabetol Date: 2013-04-15 Impact factor: 9.951
Authors: Felipe Gonçalves Dos Santos de Sá; Ana Paula Lima-Leopoldo; Bruno Barcellos Jacobsen; Artur Junio Togneri Ferron; Wagner Muller Estevam; Dijon Henrique Salomé Campos; Edson Castardeli; Márcia Regina Holanda da Cunha; Antonio Carlos Cicogna; André Soares Leopoldo Journal: Arq Bras Cardiol Date: 2015-10-27 Impact factor: 2.000
Authors: Silvio A Oliveira-Junior; Paula F Martinez; Danielle M Guizoni; Dijon H S Campos; Tiago Fernandes; Edilamar M Oliveira; Marina P Okoshi; Katashi Okoshi; Carlos R Padovani; Antonio C Cicogna Journal: PLoS One Date: 2014-01-23 Impact factor: 3.240