OBJECTIVE: The aim of this study was to assess the effect of cigarette smoke inhalation (CSI) on gene expression in alveolar bone healing sites. STUDY DESIGN: Wistar rats were randomly assigned to the groups: control [animals not exposed to CSI (n = 20)] and test [animals exposed to CSI, starting 3 days before teeth extraction and maintained until killing them (n = 20)]. First mandibular molars were bilaterally extracted, and the expression of alkaline phosphatase, bone morphogenetic protein (BMP) 2 and 7, receptor activator of nuclear factor κB ligand, osteoprotegerin, and d2 isoform of vacuolar adenosine triphosphatase V(0) domain were assessed by quantitative polymerase chain reaction in the newly formed tissue in the sockets. RESULTS: Overall, data analysis demonstrated that CSI significantly affected the expression pattern of all of the studied genes except BMP-7. CONCLUSION: The expression of key genes for bone healing may be affected by CSI in tooth extraction sites.
OBJECTIVE: The aim of this study was to assess the effect of cigarette smoke inhalation (CSI) on gene expression in alveolar bone healing sites. STUDY DESIGN:Wistar rats were randomly assigned to the groups: control [animals not exposed to CSI (n = 20)] and test [animals exposed to CSI, starting 3 days before teeth extraction and maintained until killing them (n = 20)]. First mandibular molars were bilaterally extracted, and the expression of alkaline phosphatase, bone morphogenetic protein (BMP) 2 and 7, receptor activator of nuclear factor κB ligand, osteoprotegerin, and d2 isoform of vacuolar adenosine triphosphatase V(0) domain were assessed by quantitative polymerase chain reaction in the newly formed tissue in the sockets. RESULTS: Overall, data analysis demonstrated that CSI significantly affected the expression pattern of all of the studied genes except BMP-7. CONCLUSION: The expression of key genes for bone healing may be affected by CSI in tooth extraction sites.
Authors: Nina Holzer; Karl F Braun; Sabrina Ehnert; José T Egaña; Thilo L Schenck; Arne Buchholz; Lilianna Schyschka; Markus Neumaier; Steffen Benzing; Ulrich Stöckle; Thomas Freude; Andreas K Nussler Journal: Langenbecks Arch Surg Date: 2011-12-08 Impact factor: 3.445
Authors: Claire E Otero; Jacob A Noeker; Mary M Brown; Florence D M Wavreil; Wendy A Harvey; Kristen A Mitchell; Sara J Heggland Journal: J Appl Toxicol Date: 2019-01-28 Impact factor: 3.446
Authors: Karl F Braun; Sabrina Ehnert; Thomas Freude; José T Egaña; Thilo L Schenck; Arne Buchholz; Andreas Schmitt; Sebastian Siebenlist; Lilianna Schyschka; Markus Neumaier; Ulrich Stöckle; Andreas K Nussler Journal: ScientificWorldJournal Date: 2011-11-30