Literature DB >> 2057347

31P NMR characterization of terminal phosphates induced on DNA by the artificial nuclease 'Mn-TMPyP/KHSO5' in comparison with DNases I and II.

G Gasmi1, M Pasdeloup, G Pratviel, M Pitié, J Bernadou, B Meunier.   

Abstract

Phosphorus-31 NMR has been applied to the characterization of terminal phosphates on fragments of calf thymus DNA induced by three different nuclease systems: DNase I, DNase II and the artificial nuclease 'Mn-TMPyP/KHSO5'. In this last case, the oxidative damage to deoxyribose leads to two monophosphates esters (at the 3' and 5' ends) on both sides of the cleavage site. This method constitutes a promising approach to visualise the phosphate termini generated in DNA or RNA cleavage by cytotoxic drugs or chemical nucleases and provides a novel insight into the molecular aspects of their mechanism of action.

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Year:  1991        PMID： 2057347      PMCID： PMC328240          DOI： 10.1093/nar/19.11.2835

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  22 in total

1.  Metals and DNA: molecular left-handed complements.

Authors:  J K Barton
Journal:  Science       Date:  1986-08-15       Impact factor: 47.728

2.  Design of sequence-specific DNA-binding molecules.

Authors:  P B Dervan
Journal:  Science       Date:  1986-04-25       Impact factor: 47.728

Review 3.  Porphyrin photosensitization and phototherapy.

Authors:  J Moan
Journal:  Photochem Photobiol       Date:  1986-06       Impact factor: 3.421

4.  Photocleavage of DNA in the presence of synthetic water-soluble porphyrins.

Authors:  D Praseuth; A Gaudemer; J B Verlhac; I Kraljic; I Sissoëff; E Guillé
Journal:  Photochem Photobiol       Date:  1986-12       Impact factor: 3.421

5.  Porphyrin localization: a new modality for detection and therapy of tumors.

Authors:  D Kessel
Journal:  Biochem Pharmacol       Date:  1984-05-01       Impact factor: 5.858

6.  Phosphorus-31 Fourier transform nuclear magnetic resonance study of mononucleotides and dinucleotides. 1. Chemical shifts.

Authors:  P J Cozzone; O Jardetzky
Journal:  Biochemistry       Date:  1976-11-02       Impact factor: 3.162

7.  Cleavage of DNA with methidiumpropyl-EDTA-iron(II): reaction conditions and product analyses.

Authors:  R P Hertzberg; P B Dervan
Journal:  Biochemistry       Date:  1984-08-14       Impact factor: 3.162

8.  Hemin-mediated DNA strand scission.

Authors:  R L Aft; G C Mueller
Journal:  J Biol Chem       Date:  1983-10-10       Impact factor: 5.157

9.  DNA cleavage specificity of a group of cationic metalloporphyrins.

Authors:  B Ward; A Skorobogaty; J C Dabrowiak
Journal:  Biochemistry       Date:  1986-11-04       Impact factor: 3.162

10.  Studies of yeast phenylalanine-accepting transfer ribonucleic acid backbone structure in solution by phosphorus-31 nuclear magnetic resonance spectroscopy.

Authors:  P J Salemink; T Swarthof; C W Hilbers
Journal:  Biochemistry       Date:  1979-08-07       Impact factor: 3.162
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  4 in total

1.  Using molecular beacons as a sensitive fluorescence assay for enzymatic cleavage of single-stranded DNA.

Authors:  J J Li; R Geyer; W Tan
Journal:  Nucleic Acids Res       Date:  2000-06-01       Impact factor: 16.971

2.  Preferential hydroxylation by the chemical nuclease meso-tetrakis-(4-N-methylpyridiniumyl)porphyrinatomanganeseIII pentaacetate/KHSO5 at the 5' carbon of deoxyriboses on both 3' sides of three contiguous A.T base pairs in short double-stranded oligonucleotides.

Authors:  M Pitié; G Pratviel; J Bernadou; B Meunier
Journal:  Proc Natl Acad Sci U S A       Date:  1992-05-01       Impact factor: 11.205

3.  Mechanisms of DNA damage by chromium(V) carcinogens.

Authors:  R N Bose; B S Fonkeng; S Moghaddas; D Stroup
Journal:  Nucleic Acids Res       Date:  1998-04-01       Impact factor: 16.971

4.  STRUCTURE OF PORPHYRIN TPPS4 AND ITS INTERACTION WITH METAL IONS AS ELUCIDATED BY 1H NMR AND UV-VISIBLE SPECTRA.

Authors:  Zhiyan Song; Adegboye O Adeyemo; Jannie Baker; Shakeya M Traylor; Marcia L Lightfoot
Journal:  Ga J Sci       Date:  2011
  4 in total

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