AIM: To establish whether virulence factor genes vacA and cagA are present in Helicobacter pylori (H. pylori) retrieved from gastric mucosa and dental plaque in patients with dyspepsia. METHODS: Cumulative dental plaque specimens and gastric biopsies were submitted to histological examination, rapid urease test and polymerase chain reaction (PCR) assays to detect the presence of cagA and vacA polymorphisms. RESULTS: Detection of H. pylori from dental plaque and gastric biopsy samples was greater by PCR compared to histological examination and the rapid urease test. DNA from H. pylori was detected in 96% of gastric mucosa samples and in 72% of dental plaque samples. Sixty-three (89%) of 71 dental plaque samples that were H. pylori-positive also exhibited identical vacA and cagA genotypes in gastric mucosa. The most common genotype was vacAs1bm1 and cagA positive, either in dental plaque or gastric mucosa. These virulent H. pylori isolates were involved in the severity of clinical outcome. CONCLUSION: These pathogenic strains were found simultaneously in dental plaque and gastric mucosa, which suggests that gastric infection is correlated with the presence of H. pylori in the mouth.
AIM: To establish whether virulence factor genes vacA and cagA are present in Helicobacter pylori (H. pylori) retrieved from gastric mucosa and dental plaque in patients with dyspepsia. METHODS: Cumulative dental plaque specimens and gastric biopsies were submitted to histological examination, rapid urease test and polymerase chain reaction (PCR) assays to detect the presence of cagA and vacA polymorphisms. RESULTS: Detection of H. pylori from dental plaque and gastric biopsy samples was greater by PCR compared to histological examination and the rapid urease test. DNA from H. pylori was detected in 96% of gastric mucosa samples and in 72% of dental plaque samples. Sixty-three (89%) of 71 dental plaque samples that were H. pylori-positive also exhibited identical vacA and cagA genotypes in gastric mucosa. The most common genotype was vacAs1bm1 and cagA positive, either in dental plaque or gastric mucosa. These virulent H. pylori isolates were involved in the severity of clinical outcome. CONCLUSION: These pathogenic strains were found simultaneously in dental plaque and gastric mucosa, which suggests that gastric infection is correlated with the presence of H. pylori in the mouth.
Authors: Brenda J Olivier; Robert P Bond; Walda B van Zyl; Maraliese Delport; Tomas Slavik; Christopher Ziady; Jochim S Terhaar Sive Droste; Albert Lastovica; Schalk W van der Merwe Journal: J Clin Microbiol Date: 2006-02 Impact factor: 5.948
Authors: G Gasbarrini; S Pretolani; F Bonvicini; M R Gatto; E Tonelli; F Mégraud; K Mayo; G Ghironzi; G Giulianelli; M Grassi Journal: Gut Date: 1995-06 Impact factor: 23.059
Authors: Jie Wang; David S Chi; John J Laffan; Chuanfu Li; Donald A Ferguson; Peter Litchfield; Eapen Thomas Journal: Dig Dis Sci Date: 2002-08 Impact factor: 3.199
Authors: Isabel Adler; Andrea Muiño; Silvia Aguas; Laura Harada; Mariana Diaz; Adriana Lence; Mario Labbrozzi; Juan Manuel Muiño; Boris Elsner; Alejandra Avagnina; Valeria Denninghoff Journal: World J Gastroenterol Date: 2014-08-07 Impact factor: 5.742