| Literature DB >> 20570627 |
Sunil K Berwal1, R K Sreejith, Jayanta K Pal.
Abstract
The spacing between ribosome binding site (RBS) and AUG is crucial for efficient overexpression of genes when cloned in prokaryotic expression vectors. We undertook a brief study on the overexpression of genes cloned in Escherichia coli expression vectors, wherein the spacing between the RBS and the start codon was varied. SDS-PAGE and Western blot analysis indicated a high level of protein expression only in constructs where the spacing between RBS and AUG was approximately 40 nucleotides or more, despite the synthesis of the transcripts in the representative cases investigated. Copyright 2010 Elsevier Inc. All rights reserved.Entities:
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Year: 2010 PMID： 20570627 DOI： 10.1016/j.ab.2010.06.003
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365