| Literature DB >> 20569307 |
K Sivakumaran1, M-H Chen, M J Roossinck, C C Kao.
Abstract
summary The core subgenomic promoter for the initiation of Cucumber mosaic virus (CMV) RNA4A was characterized in vitro using a template-dependent RNA synthesis assay and variants of the core promoter RNAs. The minimal sequence required for specific initiation from the cytidylate (T1) used in vivo consists of 31-nucleotides (nt) 3' of T1 and a 13 nt template sequence. This 44 nt RNA was found to provide three elements that contribute to efficient initiation of RNA4A synthesis by the CMV replicase: a stem-loop secondary structure 3' of T1, a template sequence that is rich in adenylates and uridylates, and T1 in an unbase-paired sequence.Entities:
Year: 2002 PMID: 20569307 DOI: 10.1046/j.1464-6722.2001.00089.x
Source DB: PubMed Journal: Mol Plant Pathol ISSN: 1364-3703 Impact factor: 5.663