Systemic effect of Fructus Psoraleae extract on bone in mice.

Fructus Psoraleae extract is used in China for the treatment of bone diseases. The objective of the study was to investigate the systemic effect of Fructus Psoraleae extract consumption on bone histomorphology. Sixteen 8-week-old male BALB/c mice were divided into control and experimental groups. In the control group, eight mice were fed daily with distilled water. In the experimental group, eight mice were fed daily with distilled water mixed with Fructus Psoraleae extract. The mice were kept for 5 weeks and then killed. Using micro-computed tomography, 20 micro-tomographic slices with a separation of 0.25 mm were acquired to cover the proximal end of the left tibia of each mouse. Quantitative morphometry of the bone structure was performed. The results showed that consumption of Fructus Psoraleae extract significantly increased the bone volume/tissue volume ratio by 11.8%. The bone trabeculae increased by 7.1% in thickness so that the bone density was increased. To conclude, Fructus Psoraleae extract taken orally increases bone density and alters bone histomorphology.