Literature DB >> 20564179

Ribonucleoprotein immunoprecipitation (RNP-IP): a direct in vivo analysis of microRNA-targets.

Mohammad Q Hassan1, Jonathan A R Gordon, Jane B Lian, Andre J van Wijnen, Janet L Stein, Gary S Stein.   

Abstract

We have developed a novel Ribonucleoprotein Immunoprecipitation (RNP-IP) method to isolate miR-RISC complexes, associated microRNAs and target mRNAs. Our method characterizes mRNAs present in immunoprecipitates containing miR-RISC complexes that were obtained using GW182 and AGO2 antibodies. MicroRNA bound transcripts were reverse transcribed and amplified using seed sequence and 3'UTR derived primers. This flexible IP-based assay is a straightforward method to identify miRs participating in gene regulation and their cognate mRNAs in real time. J. Cell. Biochem. 110: 817-822, 2010. (c) 2010 Wiley-Liss, Inc.

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Year:  2010        PMID： 20564179      PMCID： PMC3329753          DOI： 10.1002/jcb.22562

Source DB:  PubMed          Journal:  J Cell Biochem        ISSN： 0730-2312            Impact factor:   4.429


  11 in total

1.  Identifying mRNA subsets in messenger ribonucleoprotein complexes by using cDNA arrays.

Authors:  S A Tenenbaum; C C Carson; P J Lager; J D Keene
Journal:  Proc Natl Acad Sci U S A       Date:  2000-12-19       Impact factor: 11.205

2.  Argonaute2 is the catalytic engine of mammalian RNAi.

Authors:  Jidong Liu; Michelle A Carmell; Fabiola V Rivas; Carolyn G Marsden; J Michael Thomson; Ji-Joon Song; Scott M Hammond; Leemor Joshua-Tor; Gregory J Hannon
Journal:  Science       Date:  2004-07-29       Impact factor: 47.728

Review 3.  MicroRNA function: multiple mechanisms for a tiny RNA?

Authors:  Ramesh S Pillai
Journal:  RNA       Date:  2005-12       Impact factor: 4.942

Review 4.  Repression of protein synthesis by miRNAs: how many mechanisms?

Authors:  Ramesh S Pillai; Suvendra N Bhattacharyya; Witold Filipowicz
Journal:  Trends Cell Biol       Date:  2007-01-02       Impact factor: 20.808

5.  RIP-Chip: the isolation and identification of mRNAs, microRNAs and protein components of ribonucleoprotein complexes from cell extracts.

Authors:  Jack D Keene; Jordan M Komisarow; Matthew B Friedersdorf
Journal:  Nat Protoc       Date:  2006       Impact factor: 13.491

6.  Microarray identification of FMRP-associated brain mRNAs and altered mRNA translational profiles in fragile X syndrome.

Authors:  V Brown; P Jin; S Ceman; J C Darnell; W T O'Donnell; S A Tenenbaum; X Jin; Y Feng; K D Wilkinson; J D Keene; R B Darnell; S T Warren
Journal:  Cell       Date:  2001-11-16       Impact factor: 41.582

7.  Genome-wide identification of mRNAs associated with the translational regulator PUMILIO in Drosophila melanogaster.

Authors:  André P Gerber; Stefan Luschnig; Mark A Krasnow; Patrick O Brown; Daniel Herschlag
Journal:  Proc Natl Acad Sci U S A       Date:  2006-03-13       Impact factor: 11.205

8.  Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs.

Authors:  Gunter Meister; Markus Landthaler; Agnieszka Patkaniowska; Yair Dorsett; Grace Teng; Thomas Tuschl
Journal:  Mol Cell       Date:  2004-07-23       Impact factor: 17.970

9.  Isolation of mRNA from specific tissues of Drosophila by mRNA tagging.

Authors:  Zhiyong Yang; Howard J Edenberg; Ronald L Davis
Journal:  Nucleic Acids Res       Date:  2005-10-04       Impact factor: 16.971

10.  Extensive association of functionally and cytotopically related mRNAs with Puf family RNA-binding proteins in yeast.

Authors:  André P Gerber; Daniel Herschlag; Patrick O Brown
Journal:  PLoS Biol       Date:  2004-03-16       Impact factor: 8.029
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  11 in total

Review 1.  A study of miRNAs targets prediction and experimental validation.

Authors:  Yong Huang; Quan Zou; Haitai Song; Fei Song; Ligang Wang; Guozheng Zhang; Xingjia Shen
Journal:  Protein Cell       Date:  2010-12-10       Impact factor: 14.870

2.  A network connecting Runx2, SATB2, and the miR-23a~27a~24-2 cluster regulates the osteoblast differentiation program.

Authors:  Mohammad Q Hassan; Jonathan A R Gordon; Marcio M Beloti; Carlo M Croce; Andre J van Wijnen; Janet L Stein; Gary S Stein; Jane B Lian
Journal:  Proc Natl Acad Sci U S A       Date:  2010-10-27       Impact factor: 11.205

3.  Functional screen reveals essential roles of miR-27a/24 in differentiation of embryonic stem cells.

Authors:  Yanni Ma; Nan Yao; Guang Liu; Lei Dong; Yufang Liu; Meili Zhang; Fang Wang; Bin Wang; Xueju Wei; He Dong; Lanlan Wang; Shaowei Ji; Junwu Zhang; Yangming Wang; Yue Huang; Jia Yu
Journal:  EMBO J       Date:  2014-12-17       Impact factor: 11.598

4.  MicroRNA 665 Regulates Dentinogenesis through MicroRNA-Mediated Silencing and Epigenetic Mechanisms.

Authors:  Hannah M Heair; Austin G Kemper; Bhaskar Roy; Helena B Lopes; Harunur Rashid; John C Clarke; Lubana K Afreen; Emanuela P Ferraz; Eddy Kim; Amjad Javed; Marcio M Beloti; Mary MacDougall; Mohammad Q Hassan
Journal:  Mol Cell Biol       Date:  2015-06-29       Impact factor: 4.272

5.  Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution.

Authors:  Gatikrushna Singh; Sarah M Fritz; Arnaz Ranji; Deepali Singh; Kathleen Boris-Lawrie
Journal:  J Vis Exp       Date:  2017-01-16       Impact factor: 1.355

6.  Comprehensive analysis of microRNA (miRNA) targets in breast cancer cells.

Authors:  Meiyun Fan; Raisa Krutilina; Jing Sun; Aarti Sethuraman; Chuan He Yang; Zhao-Hui Wu; Junming Yue; Lawrence M Pfeffer
Journal:  J Biol Chem       Date:  2013-08-06       Impact factor: 5.157

7.  Characterization of extracellular circulating microRNA.

Authors:  Andrey Turchinovich; Ludmila Weiz; Anne Langheinz; Barbara Burwinkel
Journal:  Nucleic Acids Res       Date:  2011-05-24       Impact factor: 16.971

8.  TLR4/NF-κB-responsive microRNAs and their potential target genes: a mouse model of skeletal muscle ischemia-reperfusion injury.

Authors:  Johnson Chia-Shen Yang; Shao-Chun Wu; Cheng-Shyuan Rau; Yi-Chun Chen; Tsu-Hsiang Lu; Yi-Chan Wu; Siou-Ling Tzeng; Chia-Jung Wu; Ching-Hua Hsieh
Journal:  Biomed Res Int       Date:  2015-01-26       Impact factor: 3.411

9.  Detection of circulating microRNAs with Ago2 complexes to monitor the tumor dynamics of colorectal cancer patients during chemotherapy.

Authors:  Tomokazu Fuji; Yuzo Umeda; Akihiro Nyuya; Fumitaka Taniguchi; Takashi Kawai; Kazuya Yasui; Toshiaki Toshima; Kazuhiro Yoshida; Toshiyoshi Fujiwara; Ajay Goel; Takeshi Nagasaka
Journal:  Int J Cancer       Date:  2019-01-07       Impact factor: 7.396

10.  An RNA-Binding Protein, Hu-antigen R, in Pancreatic Cancer Epithelial to Mesenchymal Transition, Metastasis, and Cancer Stem Cells.

Authors:  Ruochen Dong; Ping Chen; Kishore Polireddy; Xiaoqing Wu; Tao Wang; Remya Ramesh; Dan A Dixon; Liang Xu; Jeffrey Aubé; Qi Chen
Journal:  Mol Cancer Ther       Date:  2020-09-02       Impact factor: 6.261
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