S F van Vuuren1, D Naidoo. 1. Department of Pharmacy and Pharmacology, Faculty of Health Sciences, University of Witwatersrand, Parktown, South Africa. Sandy.vanvuuren@wits.ac.za
Abstract
AIM OF THE STUDY: Eighteen plants were assessed for antimicrobial activity against pathogens associated with prevalent urogenital/sexually transmitted infections. Plant selection was based on information obtained from the ethnobotanical literature. MATERIALS AND METHODS: Dried plant material was submerged in a 1:1 mixture of methanol and dichloromethane for 24 h. Aqueous extracts were prepared by submerging dried plant material in sterile distilled water for 24 h followed by lyophilization. Essential oils were distilled from the two aromatic plant species (Tarchonanthus camphoratus and Croton gratissimus). Antimicrobial activity was assessed using the micro-well minimum inhibitory concentration (MIC) assay with specific modifications to facilitate fastidious growth of pathogens. RESULTS: Tarchonanthus camphoratus (solvent extract) showed the most significant broad spectrum activity with MIC values ranging between 0.50 and 0.70 mg/ml against five of the six pathogens tested. Other noteworthy activity was found for Hypericum aethiopicum (root) at 0.3 mg/ml (Neisseria gonorrhoeae).Polygala fruticosa and the solvent root extract of Hypericum aethiopicum showed highest sensitivities towards Gardnerella vaginalis at 0.2 mg/ml. Efficacy of the solvent extracts against the pathogen Oligella ureolytica demonstrated antimicrobial activity (MIC values <or=1.0 mg/ml) for seven plant species. The highest activity noted against Ureaplasma urealyticum was for Psidium guajava (solvent extract) at 0.8 mg/ml. In general the aqueous extracts displayed mostly poor anti-STI activity. The most noteworthy susceptibility for the aqueous extracts was noted with the plant extract Syzygium cordatum (MIC value 0.1 mg/ml against Candida albicans). The most noteworthy activity for the essential oils was observed for Tarchonanthus camphoratus (0.8 mg/ml) against Oligella ureolytica. CONCLUSIONS: Antimicrobial activity was observed for a number of the plant samples against at least one or more pathogen, thus validating the ethnobotanical use as an anti-infective to treat sexually transmitted diseases. Copyright 2010 Elsevier Ireland Ltd. All rights reserved.
AIM OF THE STUDY: Eighteen plants were assessed for antimicrobial activity against pathogens associated with prevalent urogenital/sexually transmitted infections. Plant selection was based on information obtained from the ethnobotanical literature. MATERIALS AND METHODS: Dried plant material was submerged in a 1:1 mixture of methanol and dichloromethane for 24 h. Aqueous extracts were prepared by submerging dried plant material in sterile distilled water for 24 h followed by lyophilization. Essential oils were distilled from the two aromatic plant species (Tarchonanthus camphoratus and Croton gratissimus). Antimicrobial activity was assessed using the micro-well minimum inhibitory concentration (MIC) assay with specific modifications to facilitate fastidious growth of pathogens. RESULTS:Tarchonanthus camphoratus (solvent extract) showed the most significant broad spectrum activity with MIC values ranging between 0.50 and 0.70 mg/ml against five of the six pathogens tested. Other noteworthy activity was found for Hypericum aethiopicum (root) at 0.3 mg/ml (Neisseria gonorrhoeae).Polygala fruticosa and the solvent root extract of Hypericum aethiopicum showed highest sensitivities towards Gardnerella vaginalis at 0.2 mg/ml. Efficacy of the solvent extracts against the pathogen Oligella ureolytica demonstrated antimicrobial activity (MIC values <or=1.0 mg/ml) for seven plant species. The highest activity noted against Ureaplasma urealyticum was for Psidium guajava (solvent extract) at 0.8 mg/ml. In general the aqueous extracts displayed mostly poor anti-STI activity. The most noteworthy susceptibility for the aqueous extracts was noted with the plant extract Syzygium cordatum (MIC value 0.1 mg/ml against Candida albicans). The most noteworthy activity for the essential oils was observed for Tarchonanthus camphoratus (0.8 mg/ml) against Oligella ureolytica. CONCLUSIONS: Antimicrobial activity was observed for a number of the plant samples against at least one or more pathogen, thus validating the ethnobotanical use as an anti-infective to treat sexually transmitted diseases. Copyright 2010 Elsevier Ireland Ltd. All rights reserved.
Authors: Balungile Madikizela; Ashwell Rungano Ndhlala; Jeffrey Franklin Finnie; Johannes Van Staden Journal: Evid Based Complement Alternat Med Date: 2013-03-05 Impact factor: 2.629