OBJECTIVES: To study membrane proteins modifications in Senescent Red Blood Cells (SeRBC). DESIGN AND METHODS: SeRBC were obtained on Percoll gradients. Membrane proteins were analyzed by SDS-PAGE, band 3 by immunoblotting, and protein oxidation by measuring the carbonyl groups. RESULTS: Densitometric analysis showed no change in SeRBC while an increase in band 3 and its degradation products was found. An increase of protein oxidation level was found in SeRBC. CONCLUSIONS: These findings provide further experimental evidence about protein modifications occurring during the RBC lifespan. Copyright (c) 2010 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
OBJECTIVES: To study membrane proteins modifications in Senescent Red Blood Cells (SeRBC). DESIGN AND METHODS: SeRBC were obtained on Percoll gradients. Membrane proteins were analyzed by SDS-PAGE, band 3 by immunoblotting, and protein oxidation by measuring the carbonyl groups. RESULTS: Densitometric analysis showed no change in SeRBC while an increase in band 3 and its degradation products was found. An increase of protein oxidation level was found in SeRBC. CONCLUSIONS: These findings provide further experimental evidence about protein modifications occurring during the RBC lifespan. Copyright (c) 2010 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.