OBJECTIVE: To investigate the presence of Helicobacter pylori in a Colombian population. MATERIAL AND METHODS: Gastric biopsies from 60 patients with benign gastric pathologies were submitted to histopathological examination and genotyped by PCR through amplification of babA2 and iceA genes. RESULTS: The presence of H. pylori was demonstrated in 78.3%, 63.3% and 66.7% of the samples after Giemsa staining, andH. pylori 16S DNAr and iceA gene amplification, respectively. In addition, H. pylori babA2 positive was found infecting 7 patients. Among the iceA positive samples, 35% were identified as iceAl, 47.5% as iceA2 and 17.5% contained iceAl and iceA2 or different iceA2 types. This work allowed detection and genotyping of H. pylori, demonstrating a low proportion of patients carrying strains babA2 positive and an iceA genotype distribution according to previous reports. No association was found between the presence of babA2 and iceA genes and ulcer disease.
OBJECTIVE: To investigate the presence of Helicobacter pylori in a Colombian population. MATERIAL AND METHODS: Gastric biopsies from 60 patients with benign gastric pathologies were submitted to histopathological examination and genotyped by PCR through amplification of babA2 and iceA genes. RESULTS: The presence of H. pylori was demonstrated in 78.3%, 63.3% and 66.7% of the samples after Giemsa staining, andH. pylori 16S DNAr and iceA gene amplification, respectively. In addition, H. pylori babA2 positive was found infecting 7 patients. Among the iceA positive samples, 35% were identified as iceAl, 47.5% as iceA2 and 17.5% contained iceAl and iceA2 or different iceA2 types. This work allowed detection and genotyping of H. pylori, demonstrating a low proportion of patients carrying strains babA2 positive and an iceA genotype distribution according to previous reports. No association was found between the presence of babA2 and iceA genes and ulcer disease.
Authors: Miguel Angel Aguilar-Luis; Fernando Palacios-Cuervo; Fátima Espinal-Reyes; Andrea Calderón-Rivera; Saúl Levy-Blitchtein; Carlos Palomares-Reyes; Wilmer Silva-Caso; Victor Zavaleta-Gavidia; Jorge Bazán-Mayra; Angela Cornejo-Tapia; Juana Del Valle-Mendoza; Luis J Del Valle Journal: BMC Res Notes Date: 2018-11-14