Mammalian cell transfection: the present and the future.

Transfection is a powerful analytical tool enabling study of the function of genes and gene products in cells. The transfection methods are broadly classified into three groups; biological, chemical, and physical. These methods have advanced to make it possible to deliver nucleic acids to specific subcellular regions of cells by use of a precisely controlled laser-microscope system. The combination of point-directed transfection and mRNA transfection is a new way of studying the function of genes and gene products. However, each method has its own advantages and disadvantages so the optimum method depends on experimental design and objective.

Introduction

Transfection is a procedure that introduces foreign nucleic acids into cells to produce genetically modified cells. Transfection is a powerful analytical tool for study of gene function and regulation and protein function. The introduced genetic materials (DNAs and RNAs) exist in cells either stably or transiently depending on the nature of the genetic materials [1]. For stable transfection, introduced genetic materials that usually have a marker gene for selection (transgenes) are integrated into the host genome and sustain transgene expression even after host cells replicate (Fig. 1a) [2]. In contrast with stably transfected genes, transiently transfected genes are only expressed for a limited period of time and are not integrated into the genome (Fig. 1b) [1]. Transiently transfected genetic materials can be lost by environmental factors and cell division, so the choice of stable or transient transfection depends on the objective of the experiment. The main purpose of transfection is to study the function of genes or gene products, by enhancing or inhibiting specific gene expression in cells, and to produce recombinant proteins in mammalian cells [3]. Examples are: gene therapy delivering a gene of interest into cells to cure a disease or improve symptoms; induced pluripotent stem cell (iPS cell) generation by transfecting three or four transcription factors; small interference RNA (siRNA) knock-down procedures; and production of human tissue plasminogen activator in immortalized Chinese hamster ovary (CHO) cells for therapeutic purpose [3-6].

Fig. 1

Schematic diagrams of two different transfections. (a) Stable transfection. Foreign DNA (red wave) is delivered to nucleus by passage through the cell and nuclear membranes. Foreign DNA is integrated into the host genome (black wave) and expressed sustainably. (b). Transient transfection. Foreign DNA is delivered into the nucleus but is not integrated into the genome. Foreign mRNA (blue wave) is also delivered into the cytosol, where it is translated. Hexagons are expressed proteins from transfected nucleic acids. Black arrows indicate delivery of foreign nucleic acids

Methodological diversity

Many transfection methods have been developed (Table 1). Each method uses different approaches that must be considered depending on cell type and purpose. The ideal method should have high transfection efficiency, low cell toxicity, minimal effects on normal physiology, and be easy to use and reproducible.

Table 1

Conventional transfection methods

Class	Methods	Advantages	Disadvantages	Examples	Refs.
Biological	● Virus-mediated	- High-efficiency	- Potential hazard to laboratory personnel	Herpes simplex virus, Adeno virus, Adeno-associated virus,	[4, 7–9]
		- Easy to use		Vaccinia virus, Sindbis virus
		- Effective on dissociated cells, slices, and in vivo	- Insertional mutagenesis
			- Immunogenicity
			- DNA package size limit
Chemical	● Cationic polymer	- No viral vector	- Chemical toxicity to some cell types	DEAE-dextran, polyethyleneimine, dendrimer, polybrene, calcium phosphate, lipofectin, DOTAP, lipofectamine, CTAB/DOPE, DOTMA	[10–12]
	● Calcium phosphate	- High-efficiency	- Variable transfection efficiency by cell type or condition
	● Cationic lipid	- Easy to use	- Hard to target specific cells
		- Effective on dissociated cells and slices
		- Plenty of commercially available products
		- No package size limit
Physical	● Direct injection	- Simple principle and straightforward	- Needs special instruments	Micro-needle, AFM tip, Gene Gun, Amaxa Nucleofector, phototransfection, Magnetofection	[13–25]
	● Biolistic particle delivery	- Physical relocation of nucleic acids into cell	- Vulnerable nucleic acids
	● Electroporation	- No need for vector	- Demands experimenter skill, laborious procedure
	● Laser-irradiation	- Less dependent on cell type and condition
	● Sonoporation	- Single-cell transfection
	● Magnetic nanoparticle

For this discussion, the methods are broadly classified into biologically, chemically, and physically mediated methods.

Biological methods

The most commonly used method in clinical research is virus-mediated transfection, also known as transduction [4]. Virus-mediated transfection is highly efficient and it is easy to achieve sustainable transgene expression in vivo owing to the viral nature of integration into the host genome. For example, retrovirus murine leukemia virus (MLV) has been used as a viral vector to establish sustainable transgene expression in humans [7, 8]. MLV integrates its DNA into the host genome and the integrated DNA is expressed in the host. The integrated MLV DNA replicates as the host genome does. Consequently it segregates into daughter cells, which enables sustainable transgene expression.

The major drawbacks of virus-mediated transfection are immunogenicity and cytotoxicity. Introduction of a viral vector may cause an inflammatory reaction and an insertional mutation, because viral vectors integrate into the host genome randomly, which may disrupt tumor suppressor genes, activate oncogenes, or interrupt essential genes [9]. Another disadvantage of this method is that a virus package has limited space for a foreign gene to keep infectivity. For these reasons, much effort has been made to develop non-viral transfection methods even though virus-mediated transfection is highly effective and easy to use.

Chemical methods

Chemical transfection methods are the most widely used methods in contemporary research and were the first to be used to introduce foreign genes into mammalian cells [10]. Chemical methods commonly use cationic polymer (one of the oldest chemicals used), calcium phosphate, cationic lipid (the most popular method), and cationic amino acid [10-12]. The underlying principle of chemical methods is similar. Positively charged chemicals make nucleic acid/chemical complexes with negatively charged nucleic acids. These positively charged nucleic acid/chemical complexes are attracted to the negatively charged cell membrane. The exact mechanism of how nucleic acid/chemical complexes pass through the cell membrane is unknown but it is believed that endocytosis and phagocytosis are involved in the process. Transfected DNA must be delivered to the nucleus to be expressed and again the translocation mechanism to the nucleus is not known.

The transfection efficiency of chemical methods is largely dependent on factors such as nucleic acid/chemical ratio, solution pH, and cell membrane conditions, so the process results in low transfection efficiency, especially in vivo, compared with virus-mediated methods. However, these methods have merits of relatively low cytotoxicity, no mutagenesis, no extra-carrying DNA, and no size limitation on the packaged nucleic acid. Chemical transfection efficiency also varies depending on cell type.

Physical methods

The physical transfection methods are the most recent methods and use diverse physical tools to deliver nucleic acids. The methods include direct micro injection, biolistic particle delivery, electroporation, and laser-based transfection [13]. In brief, the micro injection method directly injects nucleic acid into the cytoplasm or nucleus [14, 15]. This method delivers nucleic acids into cells but demands skill, often causes cell death, and is very labor-intensive. Biolistic particle delivery employs gold particles that conjugate with nucleic acids [16, 17]. The nucleic acid/particle conjugates are then shot into recipient cells at a high velocity (“gene gun”). This method is straightforward and reliable but it requires expensive instruments and causes physical damage to samples. Electroporation is the most widely used physical method. The exact mechanism is unknown but it is supposed that a short electrical pulse disturbs cell membranes and makes holes in the membrane through which nucleic acids can pass [18]. Because electroporation is easy and rapid, it is able to transfect a large number of cells in a short time once optimum electroporation conditions are determined. Laser-mediated transfection (also known as optoporation or phototransfection) uses a pulse laser to irradiate a cell membrane to form a transient pore [19-22]. When the laser induces a pore in the membrane, nucleic acids in the medium are transferred into the cell because of the osmotic difference between the medium and the cytosol. The laser method enables one to observe the transfecting cell and to make pores at any location on the cell. This method can be applied to very small cells, because it uses a laser, but it requires an expensive laser-microscope system. In addition to those mentioned above, there are other physical methods using ultrasound (sonoporation) and magnetic field (magnetofection) [23-25].

Transfection of RNAs

Transfecting mRNA has several merits over DNA transfection [26]. The merits include no risk of integration into the host genome, cell cycle-independent transfection efficiency, no need for immune inducible vectors, and adjustable and rapid expression. Using mRNA transfection, one can introduce any number of mRNAs into a cell, thereby overcoming overexpression of the genes. These advantages mostly originate from the fact that mRNA does not need to be located in a nucleus to be expressed. Transfected DNA must carry a host cell or tissue-specific promoter to be transcribed to mRNA and the expression level is determined by strength of the promoter. In contrast with DNA transfection, one can adjust expression levels by changing the amount of mRNA transfected and the frequency of transfection in mRNA transfection. Other strong advantages of mRNA transfection are:

transfected mRNAs can be expressed within minutes after transfection because it skips translocation to the nucleus and transcription process; and

transcriptome (population of mRNAs) can be used for transfection, which is practically difficult in DNA transfection (Fig. 2) [20, 27, 28].

Fig. 2

Micrographs of the rat hippocampal neuron lipotransfected with in-vitro-transcribed rat Gria4-GFP mRNA. (a) DIC image. (b) Fluorescence image before transfection. c, d, e, and f. Fluorescence images 2, 4, 6, and 8 h, respectively, after transfection. Note the time-dependant increases in fluorescence

For these reasons, transfecting RNA is attracting interest for therapeutic purposes [29]. However, we have to acknowledge that matured mRNA consists of five significant structures (the cap, 5′ untranslated region (5′UTR), open reading frame (ORF), 3′ untranslated region (3′UTR), and poly-A tail) and undergoes nucleoside modifications, which are important to the translation of the mRNA [30, 31]. Therefore the plasmid used for in-vitro transcription must be designed with consideration of all factors affecting stability and translational efficiency. Handling mRNA demands more caution but mRNA transfection encourages alternation with DNA transfection for many applications.

RNA interference (RNAi) is a powerful tool to knock-down specific genes and to observe consequent changes of phenotypes [6, 32]. Introduced small inhibitory RNAs (siRNA) form RNA-induced silencing complex (RISC) in the cell and the RISC inhibits the expression of target gene expression. The most common methods used to deliver siRNA are lipid/polymer-mediated delivery and virus-mediated delivery. Despite the wide use of siRNA, large efforts are still being made to develop more effective, safe, and reliable methods to deliver siRNAs into cells, because of the great potential of RNAi in clinical use to treat diseases [33]. Both relatively new transfection methods, mRNA and siRNA transfection, lead to new ways to execute cell research with their own distinctive advantages.

Single-cell transfection

Each cell has distinct gene-expression patterns even when sharing morphological similarities. Because the functions of a cell are determined by its location and time, single-cell resolution of gene expression is important to elucidate gene function. To achieve single-cell resolution of gene function, reliable single-cell transfection methods are needed. Some physical transfection methods have been applied to single-cell transfection with good results. Examples are:

micro-injections using a very small glass needle, nano-needle, femtosyringe. and atomic-force microscopy (AFM) tips [34-37];

electroporation using a micropipette filled with nucleic acids and an electric field [38]; and

phototransfection using a multi-photon laser [20].

All methods are performed under a microscope so that transfected cells can be trailed in real time. Micro-injection is straightforward and efficient but all the types of injectors actually perforate cell membranes resulting in physical damage to the cells. Single-cell electroporation efficiently delivers nucleic acids into single cells and can easily be applied in vivo. Single-cell electroporation of enhanced green fluorescence protein (EGFP) plasmid has shown the morphology and growth characteristics of a single neuron in vivo [38]. Phototransfection is the most accurate means of delivering nucleic acids (Fig. 3). Because the numbers and sizes of holes on the cell membrane can be adjusted, this method is the most suitable way of delivering population mRNAs. The additional advantage of phototransfection is that we can dictate subcellular location through which nucleic acids pass (e.g. axon or dendrite on neuron), which is not possible by electroporation. Introducing nucleic acids into a subcellular location is important for study of single polarized cells in which different cellular domains perform distinct activities. Neurons, especially, have soma, dendrites, and axons, each with a different function and localized gene expression. For example, transfection of E-26-like protein 1 (Elk-1) mRNA into dendrites of intact primary rat neurons induced cell death but introduction of Elk-1 mRNA in cell body did not cause cell death [20]. This experiment proved that localization of specific mRNA significantly altered the function of the mRNA, which was impossible to do using traditional transfection methods. The experiment could not be performed without a combination of mRNA transfection and subcellular locational transfection. Therefore, the combination of mRNA transfection and phototransfection is a powerful tool for study of gene function in single cells by virtue of point-directed delivery and immediate action of mRNA.

Fig. 3

An illustration of phototransfection. Laser beams (green flashes) create holes at specific regions of single cell (subcellular locations) and nucleic acids (red dots) are delivered into the local areas

Outlook

Transfection methods are evolving rapidly. Even within a class, many new products and technologies are launched each year with improved efficiency and less cytotoxicity. From the virus-mediated method to laser-mediated method, each method has its own advantages and disadvantages so selection of the best method depends upon the experimenter’s experimental objectives.

Future transfection technology should expand in two directions, being precise enough to transfect subcellular regions and up to whole-individual transfection. The ability to deliver foreign nucleic acids (especially mRNA) into subcellular locations (e.g. axon or dendrite) and organelles (e.g. mitochondria, golgi apparatus, or nucleus) will open an new era for genetic research because it will change how we think about and assess the function of genes in a cell. In addition to the overall gene expression profiles of a cell, the location of expressed gene products plays a crucial role in determining the function of a cell [20]. Meanwhile, safe and reliable transfection methods that can be applicable to humans are needed to establish clinical therapeutics.

In summary, transfection methodology has developed rapidly and diversely. Consequently we now have plenty of options to choose from, fitting well into our experimental or clinical needs. However, as cell research progresses, more advanced transfection technologies are still in demand.