| Literature DB >> 20546990 |
Toru Fukuda1, Shoichiro Kokabu, Satoshi Ohte, Hiroki Sasanuma, Kazuhiro Kanomata, Katsumi Yoneyama, Hitoshi Kato, Masumi Akita, Hiromi Oda, Takenobu Katagiri.
Abstract
Both BMPs and Wnts play important roles in the regulation of bone formation. We examined the molecular mechanism regulating cross-talk between BMPs and Wnts in the osteoblastic differentiation of C2C12 cells. Canonical Wnts (Wnt1 and Wnt3a) but not non-canonical Wnts (Wnt5a and Wnt11) synergistically stimulated ALP activity in the presence of BMP-4. Wnt3a and BMP-4 synergistically stimulated the expression of type I collagen and osteonectin. However, Wnt3a did not stimulate ALP activity that was induced by a constitutively active BMP receptor or Smad1. Noggin and Dkk-1 suppressed the synergistic effect of BMP-4 and Wnt3a, but Smad7 did not. Overexpression of beta-catenin did not affect BMP-4-induced ALP activity. By contrast, inhibition or stimulation of GSK3beta activity resulted in either stimulation or suppression of ALP activity, respectively, in the presence of BMP-4. Taken together, these findings suggest that BMPs and canonical Wnts may regulate osteoblastic differentiation, especially at the early stages, through a GSK3beta-dependent but beta-catenin-independent mechanism. Copyright 2010 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.Entities:
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Year: 2010 PMID： 20546990 DOI： 10.1016/j.diff.2010.05.002
Source DB: PubMed Journal: Differentiation ISSN： 0301-4681 Impact factor: 3.880