| Literature DB >> 20546712 |
Andrei V Perepelov1, Sergei D Shevelev, Bin Liu, Sof'ya N Senchenkova, Alexander S Shashkov, Lu Feng, Yuriy A Knirel, Lei Wang.
Abstract
O-Polysaccharides (O-antigens) were isolated from Escherichia coli O13, O129, and O135 and studied by chemical analyses along with 2D (1)H and (13)C NMR spectroscopy. They were found to possess a common -->2)-l-Rha-(alpha1-->2)-l-Rha-(alpha1-->3)-l-Rha-(alpha1-->3)-d-GlcNAc-(beta1--> backbone, which is a characteristic structural motif of the O-polysaccharides of Shigella flexneri types 1-5. In both the bacterial species, the backbone is decorated with lateral glucose residues or/and O-acetyl groups. In E. coli O13, a new site of glycosylation on 3-substituted Rha was revealed and the following O-polysaccharide structure was established: The structure of the E. coli O129 antigen was found to be identical to the O-antigen structure of S. flexneri type 5a specified in this work and that of E. coli O135 to S. flexneri type 4b reported earlier. Copyright (c) 2010 Elsevier Ltd. All rights reserved.Entities:
Mesh：
Substances：
Year: 2010 PMID： 20546712 DOI： 10.1016/j.carres.2010.04.023
Source DB: PubMed Journal: Carbohydr Res ISSN： 0008-6215 Impact factor: 2.104