AIMS: Glypican 3 (GPC3) has been reported to be overexpressed in yolk sac tumour (YST), but the sensitivity has not been compared with alpha-fetoprotein (AFP). YST can form numerous growth patterns and the expression of GPC3 in these patterns has not been studied. The aim was to address these aspects. METHODS AND RESULTS: Sections from testicular or ovarian YST were subjected to immunohistochemistry using GPC3 (n = 39) and AFP (n = 24). Overall immunoreactivity for each case and specific histological patterns were semiquantitatively evaluated (0-3+) and intensity of reactivity was scored (0-3). All cases expressed GPC3 (1+, 5%; 2+, 8%; 3+, 87%) with strong intensity (2.9). The majority expressed AFP (58%) but immunoreactivity was often focal (0, 42%; 1+, 33%; 2+, 25%) and intensity was low (1.0). Using GPC3, >75% of the microcystic (n = 38), macrocystic (n = 26), solid (n = 21), glandular-alveolar (n = 8), endodermal sinus (n = 7), polyvesicular vitelline (n = 5), enteric (n = 4) and micropapillary (n = 2) growth patterns displayed 2+ or 3+ positivity. CONCLUSIONS: YST can display a variety of growth patterns that can be confused with other germ cell tumour components. GPC3 detects all growth patterns tested and has a higher sensitivity for detecting YST than AFP.
AIMS: Glypican 3 (GPC3) has been reported to be overexpressed in yolk sac tumour (YST), but the sensitivity has not been compared with alpha-fetoprotein (AFP). YST can form numerous growth patterns and the expression of GPC3 in these patterns has not been studied. The aim was to address these aspects. METHODS AND RESULTS: Sections from testicular or ovarian YST were subjected to immunohistochemistry using GPC3 (n = 39) and AFP (n = 24). Overall immunoreactivity for each case and specific histological patterns were semiquantitatively evaluated (0-3+) and intensity of reactivity was scored (0-3). All cases expressed GPC3 (1+, 5%; 2+, 8%; 3+, 87%) with strong intensity (2.9). The majority expressed AFP (58%) but immunoreactivity was often focal (0, 42%; 1+, 33%; 2+, 25%) and intensity was low (1.0). Using GPC3, >75% of the microcystic (n = 38), macrocystic (n = 26), solid (n = 21), glandular-alveolar (n = 8), endodermal sinus (n = 7), polyvesicular vitelline (n = 5), enteric (n = 4) and micropapillary (n = 2) growth patterns displayed 2+ or 3+ positivity. CONCLUSIONS: YST can display a variety of growth patterns that can be confused with other germ cell tumour components. GPC3 detects all growth patterns tested and has a higher sensitivity for detecting YST than AFP.
Authors: Daniel M Berney; Ferran Algaba; Mahul Amin; Brett Delahunt; Eva Compérat; Jonathan I Epstein; Peter Humphrey; Mohammed Idrees; Antonio Lopez-Beltran; Cristina Magi-Galluzzi; Gregor Mikuz; Rodolfo Montironi; Esther Oliva; John Srigley; Victor E Reuter; Kiril Trpkov; Thomas M Ulbright; Murali Varma; Clare Verrill; Robert H Young; Ming Zhou; Lars Egevad Journal: Histopathology Date: 2015-03-17 Impact factor: 5.087
Authors: Daniel J Heidenberg; Joel H Barton; Denise Young; Michael Grinkemeyer; Isabell A Sesterhenn Journal: J Cancer Date: 2012-09-11 Impact factor: 4.207
Authors: Sigrid Marie Kraggerud; Christina E Hoei-Hansen; Sharmini Alagaratnam; Rolf I Skotheim; Vera M Abeler; Ewa Rajpert-De Meyts; Ragnhild A Lothe Journal: Endocr Rev Date: 2013-04-10 Impact factor: 19.871
Authors: Leendert H J Looijenga; Theodorus H Van der Kwast; David Grignon; Lars Egevad; Glen Kristiansen; Chia-Sui Kao; Muhammad T Idrees Journal: Am J Surg Pathol Date: 2020-07 Impact factor: 6.298