BACKGROUND AND OBJECTIVES: It is well known that provirus integration site for Moloney murine leukemia virus 1 (Pim-1) plays an essential role in the occurrence and development of tumors. Here, we investigated expression of Pim-1 in esophageal squamous cell carcinoma (ESCC) and assessed the correlation between Pim-1 level and prognosis of the patients with ESCC. METHODS: Expression of Pim-1 was investigated by RT-PCR, immunohistochemistry, and in situ hybridization methods. Kaplan-Meier statistics was used to examine the relationship between levels of Pim-1 and prognosis using log-rank test. RESULTS: The results demonstrated that ESCC tissues appeared strong expression levels of Pim-1 mRNA and protein while the normal esophageal tissues were either negative or showed only weak Pim-1 mRNA and protein levels. In addition, we found that Pim-1 expression was significantly correlated with histology grade, clinical staging, and lymph node metastasis (all P < 0.05), but not related to age and sex (all P > 0.05). Furthermore, the results of survival rates analyzed by Kaplan-Meier curve revealed that patients with high Pim-1 mRNA and protein expressions had a poorer prognosis than those with the low Pim-1 expression (P = 0.010 and 0.001, respectively). CONCLUSION: These findings demonstrate that Pim-1 may be used as molecular marker for predicting the prognosis of patients with ESCC.
BACKGROUND AND OBJECTIVES: It is well known that provirus integration site for Moloney murine leukemia virus 1 (Pim-1) plays an essential role in the occurrence and development of tumors. Here, we investigated expression of Pim-1 in esophageal squamous cell carcinoma (ESCC) and assessed the correlation between Pim-1 level and prognosis of the patients with ESCC. METHODS: Expression of Pim-1 was investigated by RT-PCR, immunohistochemistry, and in situ hybridization methods. Kaplan-Meier statistics was used to examine the relationship between levels of Pim-1 and prognosis using log-rank test. RESULTS: The results demonstrated that ESCC tissues appeared strong expression levels of Pim-1 mRNA and protein while the normal esophageal tissues were either negative or showed only weak Pim-1 mRNA and protein levels. In addition, we found that Pim-1 expression was significantly correlated with histology grade, clinical staging, and lymph node metastasis (all P < 0.05), but not related to age and sex (all P > 0.05). Furthermore, the results of survival rates analyzed by Kaplan-Meier curve revealed that patients with high Pim-1 mRNA and protein expressions had a poorer prognosis than those with the low Pim-1 expression (P = 0.010 and 0.001, respectively). CONCLUSION: These findings demonstrate that Pim-1 may be used as molecular marker for predicting the prognosis of patients with ESCC.
Authors: Susann Herzog; Matthias Alexander Fink; Kerstin Weitmann; Claudius Friedel; Stefan Hadlich; Sönke Langner; Katharina Kindermann; Tobias Holm; Andreas Böhm; Eskil Eskilsson; Hrvoje Miletic; Markus Hildner; Michael Fritsch; Silke Vogelgesang; Christoph Havemann; Christoph Alexander Ritter; Henriette Elisabeth Meyer zu Schwabedissen; Bernhard Rauch; Wolfgang Hoffmann; Heyo Klaus Kroemer; Henry Schroeder; Sandra Bien-Möller Journal: Neuro Oncol Date: 2014-08-25 Impact factor: 12.300
Authors: Andrea L Casillas; Rachel K Toth; Alva G Sainz; Neha Singh; Ankit A Desai; Andrew S Kraft; Noel A Warfel Journal: Clin Cancer Res Date: 2017-10-30 Impact factor: 12.531
Authors: Jin H Song; Neha Singh; Libia A Luevano; Sathish K R Padi; Koichi Okumura; Virginie Olive; Stephen M Black; Noel A Warfel; David W Goodrich; Andrew S Kraft Journal: Mol Cancer Ther Date: 2018-09-06 Impact factor: 6.261