Rapid temperature-programmed separation and retention prediction on a novel packed-capillary column in gas chromatography.

Novel packed-capillary columns for gas chromatography were developed with a thin-wall stainless-steel capillary of 1.0 mm i.d.; and rapid temperature-programmed separations have been carried out after a basic evaluation concerning the compatibility of these columns to the temperature program. With a numerical integration method, the retention of several test analytes during temperature-programmed elution was successfully estimated. In order to confirm the suitability of the packed-capillary columns to relatively fast temperature programming up to 40 degrees C/min, theoretically predicted retention data were compared with that actually measured. The results suggested a good separation performance of the newly developed packed-capillary columns as a particle-packed column conventionally used. Also, the compatibility to a rapid temperature-programmed operation was quite satisfactory for almost all of the separations currently done in typical analytical laboratories.