Literature DB >> 20540494

Look and see if it is time to induce protein expression in Escherichia coli cultures.

K Danielle Kelley1, Lorenzo Q Olive, Arina Hadziselimovic, Charles R Sanders.   

Abstract

It is shown that Methyl Red can be used as an indicator dye that changes color in Escherichia coli culture as a result of time- and cell density-dependent bleaching by azoreductase produced by the bacteria. For cell cultures that are being used to express a recombinant protein, this phenomenon can be exploited to provide a simple visual cue that cell cultures have reached an appropriate growth phase for addition of an agent to induce protein expression, such as isopropyl thiogalactoside.

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Year:  2010        PMID： 20540494      PMCID： PMC2896908          DOI： 10.1021/bi1007194

Source DB:  PubMed          Journal:  Biochemistry        ISSN： 0006-2960            Impact factor:   3.162


  7 in total

1.  Building a thermostable membrane protein.

Authors:  Y Zhou; J U Bowie
Journal:  J Biol Chem       Date:  2000-03-10       Impact factor: 5.157

2.  Reconstitutive refolding of diacylglycerol kinase, an integral membrane protein.

Authors:  B M Gorzelle; J K Nagy; K Oxenoid; W L Lonzer; D S Cafiso; C R Sanders
Journal:  Biochemistry       Date:  1999-12-07       Impact factor: 3.162

Review 3.  The reduction of azo dyes by the intestinal microflora.

Authors:  K T Chung; S E Stevens; C E Cerniglia
Journal:  Crit Rev Microbiol       Date:  1992       Impact factor: 7.624

4.  The Escherichia coli azoreductase AzoR Is involved in resistance to thiol-specific stress caused by electrophilic quinones.

Authors:  Guangfei Liu; Jiti Zhou; Q Shiang Fu; Jing Wang
Journal:  J Bacteriol       Date:  2009-08-07       Impact factor: 3.490

Review 5.  Direct binding of cholesterol to the amyloid precursor protein: An important interaction in lipid-Alzheimer's disease relationships?

Authors:  Andrew J Beel; Masayoshi Sakakura; Paul J Barrett; Charles R Sanders
Journal:  Biochim Biophys Acta       Date:  2010-03-18

6.  Purification and initiation of structural characterization of human peripheral myelin protein 22, an integral membrane protein linked to peripheral neuropathies.

Authors:  Charles K Mobley; Jeffrey K Myers; Arina Hadziselimovic; Charles D Ellis; Charles R Sanders
Journal:  Biochemistry       Date:  2007-09-07       Impact factor: 3.162

7.  Expansion of substrate specificity and catalytic mechanism of azoreductase by X-ray crystallography and site-directed mutagenesis.

Authors:  Kosuke Ito; Masayuki Nakanishi; Woo-Cheol Lee; Yuehua Zhi; Hiroshi Sasaki; Shuhei Zenno; Kaoru Saigo; Yukio Kitade; Masaru Tanokura
Journal:  J Biol Chem       Date:  2008-03-12       Impact factor: 5.157
  7 in total
  2 in total

1.  Cloning, Characterization, Expression Analysis, and Agglutination Studies of Novel Gene Encoding β-D-Galactose, N-Acetyl-D-Glucosamine and Lactose-Binding Lectin from Rice Bean (Vigna umbellata).

Authors:  Ankur Tripathi; Vipin Hallan; Rajan Katoch
Journal:  Mol Biotechnol       Date:  2021-10-05       Impact factor: 2.695

2.  Batch production of a silk-elastin-like protein in E. coli BL21(DE3): key parameters for optimisation.

Authors:  Tony Collins; João Azevedo-Silva; André da Costa; Fernando Branca; Raul Machado; Margarida Casal
Journal:  Microb Cell Fact       Date:  2013-02-27       Impact factor: 5.328
  2 in total

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