Literature DB >> 20536728

A novel multiplex PCR method for Clostridium botulinum neurotoxin type A gene cluster typing.

Kaoru Umeda1, Yoshiyuki Seto, Tomoko Kohda, Masafumi Mukamoto, Shunji Kozaki.   

Abstract

A rapid, simple and sensitive multiplex PCR method for boNT/A gene cluster typing was developed by combining the results of BoNT/A subtype (boNT/A1 or /A2) gene detection with ha33 and/or p47 gene detection. Ten isolates associated with infant botulism in Japan were examined and divided into boNT/A gene cluster types 2 and 3 by origin (honey feeding or not) and period (1986-1987 or 1999-2007). It is suggested that this multiplex PCR method will be be useful for epidemiological studies of botulism.

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Year:  2010        PMID： 20536728     DOI： 10.1111/j.1348-0421.2010.00213.x

Source DB:  PubMed          Journal:  Microbiol Immunol        ISSN： 0385-5600            Impact factor:   1.955


  4 in total

1.  Subtyping botulinum neurotoxins by sequential multiple endoproteases in-gel digestion coupled with mass spectrometry.

Authors:  Dongxia Wang; Jakub Baudys; Jon Rees; Kristin M Marshall; Suzanne R Kalb; Bryan A Parks; Louis Nowaczyk; James L Pirkle; John R Barr
Journal:  Anal Chem       Date:  2012-05-21       Impact factor: 6.986

2.  Genome Sequence of Clostridium botulinum Strain Adk2012 Associated with a Foodborne Botulinum Case in Tottori, Japan, in 2012.

Authors:  Hiroshi Asakura; Shiori Yamamoto; Yoshika Momose; Haru Kato; Masaaki Iwaki; Keigo Shibayama
Journal:  Genome Announc       Date:  2017-08-24

3.  Application of high-density DNA resequencing microarray for detection and characterization of botulinum neurotoxin-producing clostridia.

Authors:  Jessica Vanhomwegen; Nicolas Berthet; Christelle Mazuet; Ghislaine Guigon; Tatiana Vallaeys; Rayna Stamboliyska; Philippe Dubois; Giulia C Kennedy; Stewart T Cole; Valérie Caro; Jean-Claude Manuguerra; Michel-Robert Popoff
Journal:  PLoS One       Date:  2013-06-20       Impact factor: 3.240

4.  Molecular characterization of Clostridium botulinum isolates from foodborne outbreaks in Thailand, 2010.

Authors:  Piyada Wangroongsarb; Tomoko Kohda; Chutima Jittaprasartsin; Karun Suthivarakom; Thanitchi Kamthalang; Kaoru Umeda; Pathom Sawanpanyalert; Shunji Kozaki; Kazuyoshi Ikuta
Journal:  PLoS One       Date:  2014-01-27       Impact factor: 3.240
  4 in total

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