OBJECTIVES: To study the correlation between the HLA-B*5701 allele and the single nucleotide polymorphism in HCP5 (rs2395029). PATIENTS AND METHODS: All HIV patients naive for abacavir seen at our institution between September 2007 and December 2008 were prospectively screened for HLA-B*5701. HCP5 rs2395029 genotyping was carried out by allelic discrimination using the TaqMan 5'-nuclease assay. High-resolution HLA class I typing was undertaken using sequence-specific primers. RESULTS: A total of 245 HIV patients were included in the study. A good correlation between HLA-B*5701 and HCP5 was observed (negative and positive predictive values of 100% and 93%, respectively). CONCLUSIONS: The use of HCP5 rs2395029 testing could be as useful as HLA-B*5701 typing to prevent the abacavir hypersensitivity reaction. Given that HCP5 testing is cheaper, less time-consuming and easier to perform than HLA typing, it may confidently replace the latter in clinical settings.
OBJECTIVES: To study the correlation between the HLA-B*5701 allele and the single nucleotide polymorphism in HCP5 (rs2395029). PATIENTS AND METHODS: All HIVpatients naive for abacavir seen at our institution between September 2007 and December 2008 were prospectively screened for HLA-B*5701. HCP5rs2395029 genotyping was carried out by allelic discrimination using the TaqMan 5'-nuclease assay. High-resolution HLA class I typing was undertaken using sequence-specific primers. RESULTS: A total of 245 HIVpatients were included in the study. A good correlation between HLA-B*5701 and HCP5 was observed (negative and positive predictive values of 100% and 93%, respectively). CONCLUSIONS: The use of HCP5rs2395029 testing could be as useful as HLA-B*5701 typing to prevent the abacavirhypersensitivity reaction. Given that HCP5 testing is cheaper, less time-consuming and easier to perform than HLA typing, it may confidently replace the latter in clinical settings.
Authors: Cathelijne H van der Wouden; Mandy H van Rhenen; Wafa O M Jama; Magnus Ingelman-Sundberg; Volker M Lauschke; Lidija Konta; Matthias Schwab; Jesse J Swen; Henk-Jan Guchelaar Journal: Clin Pharmacol Ther Date: 2019-06-12 Impact factor: 6.875
Authors: Simon M Lank; Brittney A Golbach; Hannah M Creager; Roger W Wiseman; Derin B Keskin; Ellis L Reinherz; Vladimir Brusic; David H O'Connor Journal: BMC Genomics Date: 2012-08-06 Impact factor: 3.969