Literature DB >> 20532887

Transcriptional effect of a calmodulin inhibitor, W-7, on the ligninolytic enzyme genes in Phanerochaete chrysosporium.

Takaiku Sakamoto1, Hironori Kitaura, Masahiko Minami, Yoichi Honda, Takashi Watanabe, Akio Ueda, Kazumi Suzuki, Toshikazu Irie.   

Abstract

We investigated the effects of a calmodulin (CaM) inhibitor, W-7, on the expression of lignin peroxidase (LiP) and manganese peroxidase (MnP) genes in Phanerochaete chrysosporium to consider the role of cam gene, which was upregulated in parallel with the total activities of LiP and MnP in our previous transcriptomic analysis. The addition of 100 μM W-7 to the fungal cultures repressed the total activities of LiP and MnP, whereas the addition of 100 μM W-5, which is a control drug of W-7, retained approximately half of them, indicating that the effect of W-7 was attributable to CaM inhibition. Real-time reverse transcription polymerase chain reaction analysis revealed that most of lip and mnp isozyme genes predicted from whole-genome data were significantly inhibited by W-7 at the transcription level (P ≤ 0.05). These results suggest that CaM has an important role for the expression of isozyme genes of LiP and MnP at the transcription level.

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Year:  2010        PMID： 20532887     DOI： 10.1007/s00294-010-0309-z

Source DB:  PubMed          Journal:  Curr Genet        ISSN： 0172-8083            Impact factor:   3.886


  43 in total

1.  Cooperative regulation of cell proliferation by calcium and calmodulin in Aspergillus nidulans.

Authors:  K P Lu; C D Rasmussen; G S May; A R Means
Journal:  Mol Endocrinol       Date:  1992-03

2.  Changes in the gene expression of the white rot fungus Phanerochaete chrysosporium due to the addition of atropine.

Authors:  Masahiko Minami; Kazumi Suzuki; Akifumi Shimizu; Tomohiro Hongo; Takaiku Sakamoto; Naoki Ohyama; Hironori Kitaura; Akiho Kusaka; Kenji Iwama; Toshikazu Irie
Journal:  Biosci Biotechnol Biochem       Date:  2009-08-07       Impact factor: 2.043

3.  Putative calmodulin-binding domains in aflatoxin biosynthesis-regulatory proteins.

Authors:  Praveen Rao Juvvadi; Subramanyam Chivukula
Journal:  Curr Microbiol       Date:  2006-04-25       Impact factor: 2.188

4.  Calmodulin gene family in potato: developmental and touch-induced expression of the mRNA encoding a novel isoform.

Authors:  D Takezawa; Z H Liu; G An; B W Poovaiah
Journal:  Plant Mol Biol       Date:  1995-02       Impact factor: 4.076

5.  Isolation of the yeast calmodulin gene: calmodulin is an essential protein.

Authors:  T N Davis; M S Urdea; F R Masiarz; J Thorner
Journal:  Cell       Date:  1986-11-07       Impact factor: 41.582

6.  Lignin Peroxidase Isozymes from Phanerochaete chrysosporium Can Be Enzymatically Dephosphorylated.

Authors:  N Rothschild; Y Hadar; C G Dosoretz
Journal:  Appl Environ Microbiol       Date:  1997-03       Impact factor: 4.792

7.  Genome sequence of the lignocellulose degrading fungus Phanerochaete chrysosporium strain RP78.

Authors:  Diego Martinez; Luis F Larrondo; Nik Putnam; Maarten D Sollewijn Gelpke; Katherine Huang; Jarrod Chapman; Kevin G Helfenbein; Preethi Ramaiya; J Chris Detter; Frank Larimer; Pedro M Coutinho; Bernard Henrissat; Randy Berka; Dan Cullen; Daniel Rokhsar
Journal:  Nat Biotechnol       Date:  2004-05-02       Impact factor: 54.908

8.  Role of Ca2+/calmodulin signaling pathway on morphological development of Candida albicans.

Authors:  Tatsuki Sato; Yukihiro Ueno; Toshihiko Watanabe; Takeshi Mikami; Tatsuji Matsumoto
Journal:  Biol Pharm Bull       Date:  2004-08       Impact factor: 2.233

9.  Involvement of Ca(2+)/calmodulin-dependent protein kinases in mycelial growth of the basidiomycetous mushroom, Coprinus cinereus.

Authors:  Isamu Kameshita; Yusuke Yamada; Tetsuyuki Nishida; Yasunori Sugiyama; Noriyuki Sueyoshi; Akira Watanabe; Yasuhiko Asada
Journal:  Biochim Biophys Acta       Date:  2007-06-08

10.  cAMP-mediated differential regulation of lignin peroxidase and manganese-dependent peroxidase production in the white-rot basidiomycete Phanerochaete chrysosporium.

Authors:  K Boominathan; C A Reddy
Journal:  Proc Natl Acad Sci U S A       Date:  1992-06-15       Impact factor: 11.205
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  3 in total

1.  Gene expression metadata analysis reveals molecular mechanisms employed by Phanerochaete chrysosporium during lignin degradation and detoxification of plant extractives.

Authors:  Ayyappa Kumar Sista Kameshwar; Wensheng Qin
Journal:  Curr Genet       Date:  2017-03-08       Impact factor: 3.886

2.  Effects of calmodulin on expression of lignin-modifying enzymes in Pleurotus ostreatus.

Authors:  Takashi Suetomi; Takaiku Sakamoto; Yoshitaka Tokunaga; Toru Kameyama; Yoichi Honda; Hisatoshi Kamitsuji; Isamu Kameshita; Kousuke Izumitsu; Kazumi Suzuki; Toshikazu Irie
Journal:  Curr Genet       Date:  2014-11-19       Impact factor: 3.886

3.  A calmodulin inhibitor, W-7 influences the effect of cyclic adenosine 3', 5'-monophosphate signaling on ligninolytic enzyme gene expression in Phanerochaete chrysosporium.

Authors:  Takaiku Sakamoto; Yuki Yao; Yoshifumi Hida; Yoichi Honda; Takashi Watanabe; Wataru Hashigaya; Kazumi Suzuki; Toshikazu Irie
Journal:  AMB Express       Date:  2012-01-24       Impact factor: 3.298
  3 in total

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