| Literature DB >> 20530887 |
Hisashi Kimoto1, Shin-ichiro Suye, Hirokazu Makishima, Jun-ichirou Arai, Sachiko Yamaguchi, Yutaka Fujii, Toshihito Yoshioka, Akira Taketo.
Abstract
Cloning of pceA, the gene of tetrachloroethene (PCE)-reductive dehalogenase, was undertaken from environmental DNA. Two genes were amplified using PCR primer deduced from pceA. Functional expression of these genes was unsuccessful in Escherichia coli, but PceA1 synthesized in vitro was enzymatically active. In recombinant E. coli, PceA1 formed a complex with host DnaK and caused filamentous growth.Entities:
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Year: 2010 PMID： 20530887 DOI： 10.1271/bbb.100027
Source DB: PubMed Journal: Biosci Biotechnol Biochem ISSN： 0916-8451 Impact factor: 2.043