Literature DB >> 20526839

Development of a SCAR marker by inter-simple sequence repeat for diagnosis of dwarf bunt of wheat and detection of Tilletia controversa KüHN.

L Gao1, W Q Chen, T G Liu.   

Abstract

Dwarf bunt of wheat, caused by Tilletia controversa KüHN: , is a destructive disease on wheat as well as an important internationally quarantined disease in many countries. The primer ISSR818 generated a polymorphic pattern displaying a 867-bp DNA fragment specific for T. controversa. The marker was converted into a sequence characterized amplified region (SCAR), and specific primers (TCKSF3/TCKSR3) designed for use in PCR detection assays; they amplified a unique DNA fragment in all isolates of T. controversa but not in the related pathogens. The detection limit with the primer set (TCKSF3/TCKSR3) was 5 ng of DNA which could be obtained from 5.5 microg of teliospores in a 25-microL PCR reaction mixture.

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Year:  2010        PMID： 20526839     DOI： 10.1007/s12223-010-0038-1

Source DB:  PubMed          Journal:  Folia Microbiol (Praha)        ISSN： 0015-5632            Impact factor:   2.099


  22 in total

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Authors:  Milee Agarwal; Neeta Shrivastava; Harish Padh
Journal:  Plant Cell Rep       Date:  2008-02-02       Impact factor: 4.570

2.  DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.

Authors:  J G Williams; A R Kubelik; K J Livak; J A Rafalski; S V Tingey
Journal:  Nucleic Acids Res       Date:  1990-11-25       Impact factor: 16.971

3.  AFLP: a new technique for DNA fingerprinting.

Authors:  P Vos; R Hogers; M Bleeker; M Reijans; T van de Lee; M Hornes; A Frijters; J Pot; J Peleman; M Kuiper
Journal:  Nucleic Acids Res       Date:  1995-11-11       Impact factor: 16.971

4.  Application of inter simple sequence repeat (ISSR) markers to plant genetics.

Authors:  I D Godwin; E A Aitken; L W Smith
Journal:  Electrophoresis       Date:  1997-08       Impact factor: 3.535

5.  Simple sequence repeat markers distinguish among morphotypes of Sphaeropsis sapinea.

Authors:  T Burgess; M J Wingfield; B W Wingfield
Journal:  Appl Environ Microbiol       Date:  2001-01       Impact factor: 4.792

6.  DWARF BUNT: politics, identification, and biology.

Authors:  D E Mathre
Journal:  Annu Rev Phytopathol       Date:  1996       Impact factor: 13.078

7.  Use of inter-simple sequence repeats and amplified fragment length polymorphisms to analyze genetic relationships among small grain-infecting species of ustilago.

Authors:  J G Menzies; G Bakkeren; F Matheson; J D Procunier; S Woods
Journal:  Phytopathology       Date:  2003-02       Impact factor: 4.025

8.  Linkage of mating-type loci distinguishes bipolar from tetrapolar mating in basidiomycetous smut fungi.

Authors:  G Bakkeren; J W Kronstad
Journal:  Proc Natl Acad Sci U S A       Date:  1994-07-19       Impact factor: 11.205

9.  Preparation of genomic DNA for RAPD analysis from thick-walled dormant teliospores of Tilletia species.

Authors:  D R Gang; D J Weber
Journal:  Biotechniques       Date:  1995-07       Impact factor: 1.993

10.  The molecular diversity of different isolates of Beauveria bassiana (Bals.) Vuill. as assessed using intermicrosatellites (ISSRs).

Authors:  M Elena Estrada; Manuel V Camacho; César Benito
Journal:  Cell Mol Biol Lett       Date:  2006-12-28       Impact factor: 5.787
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  5 in total

1.  Development of a SCAR marker for molecular detection and diagnosis of Tilletia controversa Kühn, the causal fungus of wheat dwarf bunt.

Authors:  Li Gao; Huixin Yu; Wensu Han; Fei Gao; Taiguo Liu; Bo Liu; Xiaohui Kang; Jiguo Gao; Wanquan Chen
Journal:  World J Microbiol Biotechnol       Date:  2014-10-01       Impact factor: 3.312

2.  Analysis of Genetic Diversity and Development of SCAR Markers in a Mycogone perniciosa Population.

Authors:  Wei Wang; Xiao Li; Bingzhi Chen; Shuang Wang; Chenghuan Li; Zhiqiang Wen
Journal:  Curr Microbiol       Date:  2016-03-10       Impact factor: 2.188

3.  Development of droplet digital PCR for the detection of Tilletia laevis, which causes common bunt of wheat, based on the SCAR marker derived from ISSR and real-time PCR.

Authors:  Tongshuo Xu; Zhaoqun Yao; Jianjian Liu; Han Zhang; Ghulam Muhae Ud Din; Sifeng Zhao; Wanquan Chen; Taiguo Liu; Li Gao
Journal:  Sci Rep       Date:  2020-09-30       Impact factor: 4.379

4.  Transcriptome analysis of wheat spikes in response to Tilletia controversa Kühn which cause wheat dwarf bunt.

Authors:  Zhaoyu Ren; Jianjian Liu; Ghulam Muhae Ud Din; Han Zhang; Zhenzhen Du; Wanquan Chen; Taiguo Liu; Jianmin Zhang; Sifeng Zhao; Li Gao
Journal:  Sci Rep       Date:  2020-12-09       Impact factor: 4.379

5.  Development of the Droplet Digital PCR to Detect the Teliospores of Tilletia controversa Kühn in the Soil With Greatly Enhanced Sensitivity.

Authors:  Jianjian Liu; Chao Li; Ghulam Muhae-Ud-Din; Taiguo Liu; Wanquan Chen; Jianmin Zhang; Li Gao
Journal:  Front Microbiol       Date:  2020-01-30       Impact factor: 5.640
  5 in total

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