PURPOSE: To establish an in vitro culture system for mouse round spermatids that models spermiogenesis and enables the assessment of oocyte activation ability. METHODS: Round spermatids and Sertoli cells were isolated from testicular tissues of B6D2F1 male mice and co-cultured in the presence of testosterone and recombinant FSH. Cultured spermatids were examined for morphology and condensation of nuclei, fertilization and development rate, and Ca²(+) oscillation pattern after ICSI. RESULTS: The cultured spermatids elongated and resembled normal elongating spermatids in terms of both morphology and nuclear condensation. No significant differences in fertilization and development rates were observed between fresh and cultured elongating spermatids. Moreover, cultured spermatids showed similar Ca²(+) oscillation patterns to fresh elongating spermatids during an initial stage in oocyte activation. CONCLUSIONS: These data suggest that a co-culture system of spermatids and Sertoli cells, supplemented with testosterone and recombinant FSH, supports normal differentiation of round spermatids into elongating spermatids, as assessed by their morphology, nuclear condensation, and oocyte activation ability.
PURPOSE: To establish an in vitro culture system for mouse round spermatids that models spermiogenesis and enables the assessment of oocyte activation ability. METHODS: Round spermatids and Sertoli cells were isolated from testicular tissues of B6D2F1 male mice and co-cultured in the presence of testosterone and recombinant FSH. Cultured spermatids were examined for morphology and condensation of nuclei, fertilization and development rate, and Ca²(+) oscillation pattern after ICSI. RESULTS: The cultured spermatids elongated and resembled normal elongating spermatids in terms of both morphology and nuclear condensation. No significant differences in fertilization and development rates were observed between fresh and cultured elongating spermatids. Moreover, cultured spermatids showed similar Ca²(+) oscillation patterns to fresh elongating spermatids during an initial stage in oocyte activation. CONCLUSIONS: These data suggest that a co-culture system of spermatids and Sertoli cells, supplemented with testosterone and recombinant FSH, supports normal differentiation of round spermatids into elongating spermatids, as assessed by their morphology, nuclear condensation, and oocyte activation ability.
Authors: P Vanderzwalmen; H Zech; A Birkenfeld; M Yemini; G Bertin; B Lejeune; M Nijs; L Segal; A Stecher; B Vandamme; E van Roosendaal; R Schoysman Journal: Hum Reprod Date: 1997-06 Impact factor: 6.918
Authors: Sook-Young Yoon; Teru Jellerette; Ana Maria Salicioni; Hoi Chang Lee; Myung-Sik Yoo; Kevin Coward; John Parrington; Daniel Grow; Jose B Cibelli; Pablo E Visconti; Jesse Mager; Rafael A Fissore Journal: J Clin Invest Date: 2008-10-16 Impact factor: 14.808
Authors: Patricio Ventura-Juncá; Isabel Irarrázaval; Augusto J Rolle; Juan I Gutiérrez; Ricardo D Moreno; Manuel J Santos Journal: Biol Res Date: 2015-12-18 Impact factor: 5.612
Authors: Shou-Long Deng; Yan Zhang; Kun Yu; Xiu-Xia Wang; Su-Ren Chen; De-Ping Han; C Yan Cheng; Zheng-Xing Lian; Yi-Xun Liu Journal: Oncotarget Date: 2017-12-01