OBJECTIVE: The present study aimed to investigate the salivary chemokine levels in patients with primary SS (pSS) and compare them with those in patients with non-SS sicca symptoms or non-sicca controls. METHODS: Unstimulated and stimulated whole saliva samples were obtained from pSS patients (n = 30) and age- and gender-matched patients with non-SS sicca (n = 30) and non-sicca healthy controls (n = 25). Salivary CCL2, CCL3, CCL4, CXCL8 and CXCL10 levels were measured using a Luminex bead-based multiplex assay. RESULTS: Patients with pSS had significantly different distributions of salivary CCL3 (P = 0.0001 by the Kruskal-Wallis test), CCL4 (P < 0.00001), CXLC8 (P < 0.0001) and CXCL10 (P < 0.05) levels in unstimulated saliva and all chemokine levels in stimulated saliva when compared with non-SS sicca and non-sicca controls. In comparison with chemokine production rate, the CXCL8 and CXCL10 production rates were significantly higher in pSS than in non-SS sicca controls (P < 0.01 by the Mann-Whitney test). Logistic regression analyses revealed that salivary CXCL8 (P < 0.05) and CXCL10 (P < 0.05) were the significant discriminating chemokines between the pSS and non-SS sicca groups. Although CXCL8 and CXCL10 levels were not correlated with the focus scores, CXCL8 and CXCL10 levels were significantly associated with salivary gland dysfunction. CONCLUSION: These results support the notion that CXCL8 or CXCL10 chemokine plays a role in the pathogenesis of pSS.
OBJECTIVE: The present study aimed to investigate the salivary chemokine levels in patients with primary SS (pSS) and compare them with those in patients with non-SS sicca symptoms or non-sicca controls. METHODS: Unstimulated and stimulated whole saliva samples were obtained from pSSpatients (n = 30) and age- and gender-matched patients with non-SS sicca (n = 30) and non-sicca healthy controls (n = 25). Salivary CCL2, CCL3, CCL4, CXCL8 and CXCL10 levels were measured using a Luminex bead-based multiplex assay. RESULTS:Patients with pSS had significantly different distributions of salivary CCL3 (P = 0.0001 by the Kruskal-Wallis test), CCL4 (P < 0.00001), CXLC8 (P < 0.0001) and CXCL10 (P < 0.05) levels in unstimulated saliva and all chemokine levels in stimulated saliva when compared with non-SS sicca and non-sicca controls. In comparison with chemokine production rate, the CXCL8 and CXCL10 production rates were significantly higher in pSS than in non-SS sicca controls (P < 0.01 by the Mann-Whitney test). Logistic regression analyses revealed that salivary CXCL8 (P < 0.05) and CXCL10 (P < 0.05) were the significant discriminating chemokines between the pSS and non-SS sicca groups. Although CXCL8 and CXCL10 levels were not correlated with the focus scores, CXCL8 and CXCL10 levels were significantly associated with salivary gland dysfunction. CONCLUSION: These results support the notion that CXCL8 or CXCL10 chemokine plays a role in the pathogenesis of pSS.
Authors: Thomas Egerer; Lorena Martinez-Gamboa; Anja Dankof; Bruno Stuhlmüller; Thomas Dörner; Veit Krenn; Karl Egerer; Paul E Rudolph; Gerd-R Burmester; Eugen Feist Journal: Arthritis Rheum Date: 2006-05
Authors: Ana P Lopes; Cornelis P J Bekker; Maarten R Hillen; Sofie L M Blokland; Anneline C Hinrichs; Aridaman Pandit; Aike A Kruize; Timothy R D J Radstake; Joel A G van Roon Journal: Front Immunol Date: 2021-08-03 Impact factor: 7.561
Authors: K Yoshimoto; T Fujimoto; A Itaya-Hironaka; T Miyaoka; S Sakuramoto-Tsuchida; A Yamauchi; M Takeda; T Kasai; K Nakagawara; A Nonomura; S Takasawa Journal: Clin Exp Immunol Date: 2013-10 Impact factor: 4.330
Authors: M Moriyama; J-N Hayashida; T Toyoshima; Y Ohyama; S Shinozaki; A Tanaka; T Maehara; S Nakamura Journal: Clin Exp Immunol Date: 2012-07 Impact factor: 4.330