BACKGROUND: Nkx2.5 is a homeodomain-containing nuclear transcription protein that has been associated with acute T-lymphoblastic leukemia. In addition, Nkx2.5 has an essential role in cardiomyogenesis. However, the expression of Nkx2.5 in the skin has not been investigated. OBJECTIVE: In an attempt to screen the differentially regulated genes involved in keratinocyte differentiation, using a cDNA microarray, we identified Nkx2.5 as one of the transcription factors controlling the expression of proteins associated with keratinocyte differentiation. METHODS: To investigate the expression of Nkx2.5 during keratinocyte differentiation, we used a calcium-induced keratinocyte differentiation model. RESULTS: RT-PCR and Western blot analysis revealed that the expression of Nkx2.5, in cultured human epidermal keratinocytes, increased with calcium treatment in a time-dependent manner. In normal skin tissue, the expression of Nkx2.5 was detected in the nuclei of the keratinocytes in all layers of the epidermis except the basal layer by immunohistochemistry. In addition, the expression of Nkx2.5 was significantly increased in psoriasis and squamous cell carcinoma, but was barely detected in atopic dermatitis and basal cell carcinoma. CONCLUSION: These results suggest that Nkx2.5 may play a role in the change from proliferation to differentiation of keratinocytes and in the pathogenesis of skin disease with aberrant keratinocyte differentiation.
BACKGROUND:Nkx2.5 is a homeodomain-containing nuclear transcription protein that has been associated with acute T-lymphoblastic leukemia. In addition, Nkx2.5 has an essential role in cardiomyogenesis. However, the expression of Nkx2.5 in the skin has not been investigated. OBJECTIVE: In an attempt to screen the differentially regulated genes involved in keratinocyte differentiation, using a cDNA microarray, we identified Nkx2.5 as one of the transcription factors controlling the expression of proteins associated with keratinocyte differentiation. METHODS: To investigate the expression of Nkx2.5 during keratinocyte differentiation, we used a calcium-induced keratinocyte differentiation model. RESULTS: RT-PCR and Western blot analysis revealed that the expression of Nkx2.5, in cultured human epidermal keratinocytes, increased with calcium treatment in a time-dependent manner. In normal skin tissue, the expression of Nkx2.5 was detected in the nuclei of the keratinocytes in all layers of the epidermis except the basal layer by immunohistochemistry. In addition, the expression of Nkx2.5 was significantly increased in psoriasis and squamous cell carcinoma, but was barely detected in atopic dermatitis and basal cell carcinoma. CONCLUSION: These results suggest that Nkx2.5 may play a role in the change from proliferation to differentiation of keratinocytes and in the pathogenesis of skin disease with aberrant keratinocyte differentiation.
Authors: Leszek Markuszeski; Andrzej Bissinger; Iwo Janusz; Joanna Narbutt; Anna Sysa Jedrzejowska; Anna Zalewska Journal: Arch Med Res Date: 2006-11-03 Impact factor: 2.235
Authors: Chenglong Han; Don W Robinson; Monica V Hackett; L Clark Paramore; Kathy H Fraeman; Mohan V Bala Journal: J Rheumatol Date: 2006-09-01 Impact factor: 4.666
Authors: Grzegorz K Przybylski; Willem A Dik; Piotr Grabarczyk; Jens Wanzeck; Paulina Chudobska; Kacper Jankowski; Anne von Bergh; Jacques J M van Dongen; Christian A Schmidt; Anton W Langerak Journal: Haematologica Date: 2006-03 Impact factor: 9.941
Authors: Doff B McElhinney; Elizabeth Geiger; Joshua Blinder; D Woodrow Benson; Elizabeth Goldmuntz Journal: J Am Coll Cardiol Date: 2003-11-05 Impact factor: 24.094
Authors: Seung Ju Back; Myung Im; Kyung Cheol Sohn; Dae Kyoung Choi; Ge Shi; Nam Ji Jeong; Young Lee; Young Joon Seo; Chang Deok Kim; Jeung Hoon Lee Journal: Ann Dermatol Date: 2012-07-25 Impact factor: 1.444
Authors: Meng Du; Xiaojing Wang; Xin Tan; Xiangrao Li; Dandan Huang; Kun Huang; Liu Yang; Fengxiao Zhang; Yan Wang; Dan Huang; Kai Huang Journal: J Am Heart Assoc Date: 2016-12-19 Impact factor: 5.501
Authors: Ricardo Cortez Cardoso Penha; Luisa Aguirre Buexm; Fabiana Resende Rodrigues; Taciana Padilha de Castro; Maria Carolina S Santos; Rodrigo Soares Fortunato; Denise P Carvalho; Luciene C Cardoso-Weide; Andrea C F Ferreira Journal: BMC Cancer Date: 2018-05-02 Impact factor: 4.430