BACKGROUND: Little information is available on eosinophil activation and cytokine/chemokine responses in childhood asthma, thus we examined serum eosinophil cationic protein (ECP) and 27 types of cytokines/chemokines in acute exacerbation of asthma (acute asthma) and stable asthma. METHODS: We determined peripheral eosinophil count, and the serum levels of ECP and 27 types of cytokines/chemokines (IL-1 beta, IL-1 ra, IL-2, -4, -5, -6, -7, -8, -9, -10, -12, -13, -15 and -17, IFN-gamma, IP-10, TNF-alpha, GM-CSF, G-CSF, MCP-1, MIP-1 alpha and -1 beta, eotaxin, RANTES, PDGF-bb, FGF basic and VEGF) using a multiplex bead-based assay in 85 acute and 79 stable asthma patients, and 14 controls. We also examined the effects of systemic corticosteroids on these responses in acute asthma. RESULTS: The serum levels of ECP, IL-5, -6, -8 and -10, G-CSF, MCP-1, IL-1 ra and IP-10 were significantly elevated in acute compared with stable asthma. Similarly, serum levels of ECP, IL-5 and IP-10 were significantly higher in acute asthma than in controls. Furthermore, in the acute phase, elevated serum levels of ECP, IL-5, IL-6, IL-1 ra and IP-10, but not IL-8, IL-10, G-CSF and MCP-1 were significantly reduced after treatments that included systemic corticosteroids. CONCLUSION: Eosinophil activation could be induced by acute exacerbation of childhood asthma. (c) 2010 S. Karger AG, Basel.
BACKGROUND: Little information is available on eosinophil activation and cytokine/chemokine responses in childhood asthma, thus we examined serum eosinophil cationic protein (ECP) and 27 types of cytokines/chemokines in acute exacerbation of asthma (acute asthma) and stable asthma. METHODS: We determined peripheral eosinophil count, and the serum levels of ECP and 27 types of cytokines/chemokines (IL-1 beta, IL-1 ra, IL-2, -4, -5, -6, -7, -8, -9, -10, -12, -13, -15 and -17, IFN-gamma, IP-10, TNF-alpha, GM-CSF, G-CSF, MCP-1, MIP-1 alpha and -1 beta, eotaxin, RANTES, PDGF-bb, FGF basic and VEGF) using a multiplex bead-based assay in 85 acute and 79 stable asthmapatients, and 14 controls. We also examined the effects of systemic corticosteroids on these responses in acute asthma. RESULTS: The serum levels of ECP, IL-5, -6, -8 and -10, G-CSF, MCP-1, IL-1 ra and IP-10 were significantly elevated in acute compared with stable asthma. Similarly, serum levels of ECP, IL-5 and IP-10 were significantly higher in acute asthma than in controls. Furthermore, in the acute phase, elevated serum levels of ECP, IL-5, IL-6, IL-1 ra and IP-10, but not IL-8, IL-10, G-CSF and MCP-1 were significantly reduced after treatments that included systemic corticosteroids. CONCLUSION: Eosinophil activation could be induced by acute exacerbation of childhood asthma. (c) 2010 S. Karger AG, Basel.
Authors: Matthew S Godwin; Kristen M Reeder; Jaleesa M Garth; Jonathan P Blackburn; MaryJane Jones; Zhihong Yu; Sadis Matalon; Annette T Hastie; Deborah A Meyers; Chad Steele Journal: JCI Insight Date: 2019-11-01
Authors: Dhiren F Patel; Teresa Peiró; Nicoletta Bruno; Juho Vuononvirta; Samia Akthar; Franz Puttur; Chloe J Pyle; Kornelija Suveizdytė; Simone A Walker; Aran Singanayagam; Leo M Carlin; Lisa G Gregory; Clare M Lloyd; Robert J Snelgrove Journal: Sci Immunol Date: 2019-11-08
Authors: Mariam Ba; Shanti Rawat; Ronna Lao; Marilyn Grous; Michael Salmon; Andrew J Halayko; William T Gerthoffer; Cherie A Singer Journal: Pulm Pharmacol Ther Date: 2018-09-08 Impact factor: 3.282