Literature DB >> 20522329

A survey of quantitative real-time polymerase chain reaction internal reference genes for expression studies in Brassica napus.

Xue Chen1, Martin Truksa, Saleh Shah, Randall J Weselake.   

Abstract

Eight reference genes of Brassica napus were evaluated using quantitative real-time polymerase chain reaction (qRT-PCR) data, focusing on vegetative tissues and developing embryos. Analyses of expression stability indicated that UP1, UBC9, UBC21, and TIP41 were the top four choices as stably expressed reference genes for vegetative tissues, whereas ACT7, UBC21, TIP41, and PP2A were the top four choices for maturing embryos. In addition, radiolabeling of overall messenger RNA (mRNA) of maturing embryos indicated that the expression patterns of the top four ranked reference genes reflected the overall mRNA content changes in maturing embryos. Crown Copyright 2010. Published by Elsevier Inc. All rights reserved.

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Year:  2010        PMID： 20522329     DOI： 10.1016/j.ab.2010.05.032

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  42 in total

1.  Comprehensive selection of reference genes for quantitative gene expression analysis during seed development in Brassica napus.

Authors:  Ronei Dorneles Machado; Ana Paula Christoff; Guilherme Loss-Morais; Márcia Margis-Pinheiro; Rogério Margis; Ana Paula Körbes
Journal:  Plant Cell Rep       Date:  2015-02-27       Impact factor: 4.570

2.  Transparent testa16 plays multiple roles in plant development and is involved in lipid synthesis and embryo development in canola.

Authors:  Wei Deng; Guanqun Chen; Fred Peng; Martin Truksa; Crystal L Snyder; Randall J Weselake
Journal:  Plant Physiol       Date:  2012-07-30       Impact factor: 8.340

3.  A guideline to family-wide comparative state-of-the-art quantitative RT-PCR analysis exemplified with a Brassicaceae cross-species seed germination case study.

Authors:  Kai Graeber; Ada Linkies; Andrew T A Wood; Gerhard Leubner-Metzger
Journal:  Plant Cell       Date:  2011-06-10       Impact factor: 11.277

4.  Reduction of antinutritional glucosinolates in Brassica oilseeds by mutation of genes encoding transporters.

Authors:  Hussam Hassan Nour-Eldin; Svend Roesen Madsen; Steven Engelen; Morten Egevang Jørgensen; Carl Erik Olsen; Jonathan Sonne Andersen; David Seynnaeve; Thalia Verhoye; Rudy Fulawka; Peter Denolf; Barbara Ann Halkier
Journal:  Nat Biotechnol       Date:  2017-03-13       Impact factor: 54.908

5.  Three homologous genes encoding sn-glycerol-3-phosphate acyltransferase 4 exhibit different expression patterns and functional divergence in Brassica napus.

Authors:  Xue Chen; Martin Truksa; Crystal L Snyder; Aliaa El-Mezawy; Saleh Shah; Randall J Weselake
Journal:  Plant Physiol       Date:  2010-12-20       Impact factor: 8.340

6.  Selection of suitable reference genes for quantitative real-time PCR gene expression analysis in Mulberry (Morus alba L.) under different abiotic stresses.

Authors:  Pawan Shukla; Ramesha A Reddy; Kangayam M Ponnuvel; Gulab Khan Rohela; Aftab A Shabnam; M K Ghosh; Rakesh Kumar Mishra
Journal:  Mol Biol Rep       Date:  2019-01-29       Impact factor: 2.316

7.  Selection of reference genes for quantitative reverse-transcription polymerase chain reaction normalization in Brassica napus under various stress conditions.

Authors:  Zheng Wang; Yu Chen; Hedi Fang; Haifeng Shi; Keping Chen; Zhiyan Zhang; Xiaoli Tan
Journal:  Mol Genet Genomics       Date:  2014-04-27       Impact factor: 3.291

8.  Defining reference genes for qPCR normalization to study biotic and abiotic stress responses in Vigna mungo.

Authors:  Anirban Kundu; Anju Patel; Amita Pal
Journal:  Plant Cell Rep       Date:  2013-07-19       Impact factor: 4.570

9.  Selection of reference genes for quantitative real-time PCR in bamboo (Phyllostachys edulis).

Authors:  Chunjie Fan; Jinmin Ma; Qirong Guo; Xiaotie Li; Hui Wang; Mengzhu Lu
Journal:  PLoS One       Date:  2013-02-20       Impact factor: 3.240

10.  Selection of reliable reference genes for gene expression studies using real-time PCR in tung tree during seed development.

Authors:  Xiaojiao Han; Mengzhu Lu; Yicun Chen; Zhiyong Zhan; Qinqin Cui; Yangdong Wang
Journal:  PLoS One       Date:  2012-08-17       Impact factor: 3.240
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