Literature DB >> 2052011

Effect of exogenous DNA fragments on human cell extract-mediated DNA repair synthesis.

M Biggerstaff1, P Robins, D Coverley, R D Wood.   

Abstract

Extracts from HeLa cells were used to study the susceptibility of repair synthesis in UV-irradiated plasmid DNA to inhibition by exogenously added nucleic acid. Purified DNA restriction fragments have little inhibitory effect on repair synthesis. However, activated calf thymus DNA fragments, genomic DNA fragments in cell extracts, and sonicated plasmid DNA all inhibited repair synthesis. Degraded DNA fragments arising from E. coli during bacterial plasmid purification were found to be particularly inhibitory. tRNA is not a potent inhibitor of in vitro repair synthesis. In order to observe efficient DNA repair synthesis mediated by human cell extracts, it is essential to prepare highly purified closed circular plasmid DNA, and we describe a reliable method for doing so.

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Year:  1991        PMID： 2052011     DOI： 10.1016/0921-8777(91)90059-x

Source DB:  PubMed          Journal:  Mutat Res        ISSN： 0027-5107            Impact factor:   2.433


  17 in total

1.  Reduced RNA polymerase II transcription in extracts of cockayne syndrome and xeroderma pigmentosum/Cockayne syndrome cells.

Authors:  G L Dianov; J F Houle; N Iyer; V A Bohr; E C Friedberg
Journal:  Nucleic Acids Res       Date:  1997-09-15       Impact factor: 16.971

2.  The post-incision steps of the DNA base excision repair pathway in Escherichia coli: studies with a closed circular DNA substrate containing a single U:G base pair.

Authors:  M Sandigursky; G A Freyer; W A Franklin
Journal:  Nucleic Acids Res       Date:  1998-03-01       Impact factor: 16.971

3.  A repair competition assay to assess recognition by human nucleotide excision repair.

Authors:  M T Hess; D Gunz; H Naegeli
Journal:  Nucleic Acids Res       Date:  1996-03-01       Impact factor: 16.971

4.  The Werner syndrome protein is involved in RNA polymerase II transcription.

Authors:  A S Balajee; A Machwe; A May; M D Gray; J Oshima; G M Martin; J O Nehlin; R Brosh; D K Orren; V A Bohr
Journal:  Mol Biol Cell       Date:  1999-08       Impact factor: 4.138

5.  Properties of damage-dependent DNA incision by nucleotide excision repair in human cell-free extracts.

Authors:  P Calsou; B Salles
Journal:  Nucleic Acids Res       Date:  1994-11-25       Impact factor: 16.971

6.  Xenopus egg lysates repair heat-generated DNA nicks with an average patch size of 36 nucleotides.

Authors:  L Höfferer; K H Winterhalter; F R Althaus
Journal:  Nucleic Acids Res       Date:  1995-04-25       Impact factor: 16.971

7.  Generation of single-nucleotide repair patches following excision of uracil residues from DNA.

Authors:  G Dianov; A Price; T Lindahl
Journal:  Mol Cell Biol       Date:  1992-04       Impact factor: 4.272

8.  Repair of damaged DNA by extracts from a xeroderma pigmentosum complementation group A revertant and expression of a protein absent in its parental cell line.

Authors:  C J Jones; J E Cleaver; R D Wood
Journal:  Nucleic Acids Res       Date:  1992-03-11       Impact factor: 16.971

9.  Repair synthesis by human cell extracts in cisplatin-damaged DNA is preferentially determined by minor adducts.

Authors:  P Calsou; P Frit; B Salles
Journal:  Nucleic Acids Res       Date:  1992-12-11       Impact factor: 16.971

10.  An intrastrand d(GpG) platinum crosslink in duplex M13 DNA is refractory to repair by human cell extracts.

Authors:  D E Szymkowski; K Yarema; J M Essigmann; S J Lippard; R D Wood
Journal:  Proc Natl Acad Sci U S A       Date:  1992-11-15       Impact factor: 11.205
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